Derivatization, or chemical structure modification, is often used in bioanalysis performed by liquid chromatography technique in order to enhance detectability or to improve the chromatographic performance for the target analytes. The derivatization process is discussed according to the analytical procedure used to achieve the reaction between the reagent and the target compounds (containing hydroxyl, thiol, amino, carbonyl and carboxyl as the main functional groups involved in derivatization). Important procedures for derivatization used in bioanalysis are in situ or based on extraction processes (liquid–liquid, solid‐phase and related techniques) applied to the biomatrix. In the review, chiral, isotope‐labeling, hydrophobicity‐tailored and post‐column derivatizations are also included, based on representative publications in the literature during the last two decades. Examples of derivatization reagents and brief reaction conditions are included, together with some bioanalytical applications and performances (chromatographic conditions, detection limit, stability and sample biomatrix).

中文翻译：

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生物分析中HPLC测定的衍生化程序及其分析性能

衍生化或化学结构修饰通常用于通过液相色谱技术进行的生物分析中，以增强目标分析物的可检测性或色谱性能。根据用于实现试剂与目标化合物（包含羟基，硫醇，氨基，羰基和羧基作为衍生化主要功能基团）之间反应的分析程序，讨论了衍生化过程。生物分析中重要的衍生化程序是就地的或基于应用于生物基质的提取过程（液-液，固相和相关技术）。在回顾中，根据近二十年来文献中的代表性出版物，还包括手性，同位素标记，疏水性定制和柱后衍生化。包括衍生化试剂和简短反应条件的示例，以及一些生物分析应用和性能（色谱条件，检测限，稳定性和样品生物基质）。