Mass cytometry is attracting significant attention for enabling spatiotemporal high‐throughput single‐cell analysis. As the first demonstration of the simultaneous detection of single‐cell proteins and untargeted metabolites, a multi‐dimensional organic mass‐cytometry system was established by a simple microfluidic chip connected to a nanoelectrospray mass spectrometer, providing useful heterogeneous information about the cells. A series of mass probes with online‐dissociated mass tags were developed, ensuring the semi‐quantification of cell‐surface proteins and the compatibility of endogenous metabolite detection at the single‐cell level. Six cell surface antigens and ≈100 metabolites from three ovarian‐cancer cell types and two breast‐cancer cell types were successfully monitored and contributed to highly sensitive and specific cell typing. Doxorubicin‐resistant cancer‐cell analysis confirmed the applications in distinguishing rare cell phenotypes. The proposed system is simple, extensible, and promising for cell typing, drug‐resistance analysis of tumor cells, and clinical diagnosis and therapy at the single‐cell level.

中文翻译：

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多维有机质量细胞术：同时分析单细胞上的蛋白质和代谢产物

大规模流式细胞术引起了时空高通量单细胞分析的广泛关注。作为同时检测单细胞蛋白质和非靶向代谢物的第一个证明，通过连接到纳米电喷雾质谱仪的简单微流控芯片建立了多维有机质谱系统，提供了有关细胞的有用的异质信息。开发了一系列具有在线分离质量标签的质量探针，以确保细胞表面蛋白的半定量以及内源性代谢物检测在单细胞水平上的兼容性。成功监测了来自三种卵巢癌细胞类型和两种乳腺癌细胞类型的六种细胞表面抗原和约100种代谢物，并有助于高度敏感和特异的细胞分型。阿霉素抗性癌细胞分析证实了在区分稀有细胞表型方面的应用。拟议的系统简单，可扩展，并且有望用于细胞分型，肿瘤细胞的耐药性分析以及单细胞水平的临床诊断和治疗。