Method development is one of the objectives of the astrophysical community for characterizing the organic matter in objects of the solar system. In this context, we report on the development of an enzyme-catalyzed stereoselective hydrolysis, inspired by the proteomics discipline, which has enabled the indirect detection of peptide sequences in extraterrestrial samples. A proof of concept has been performed on a Murchison extract. We show that our approach can successfully highlight l- and d-amino acids involved in peptide bonds. While we show that some d-amino acids must have been involved in peptide bonds, we cannot at this stage conclude on the indigenous or exogenous nature of these biopolymers. However, our strategy constitutes the first step toward direct UPLC-MS evidence of peptide sequences in extraterrestrial samples. It should thus contribute to deepening knowledge on the molecules available in the solar system, hence providing new clues about their chemical history, especially on Earth.

中文翻译：

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基于蛋白质组学的新型策略，用于研究地球外样品中的肽序列

方法开发是天文学界用来表征太阳系物体中有机物特征的目标之一。在这种情况下，我们在蛋白质组学的启发下，报告了酶催化的立体选择性水解的发展，这种水解使得能够间接检测地外样品中的肽序列。对Murchison提取物进行了概念验证。我们表明，我们的方法可以成功地突出参与肽键的l-和d-氨基酸。虽然我们表明，一些d-氨基酸必须与肽键有关，在此阶段我们不能就这些生物聚合物的固有性质或外源性质做出结论。但是，我们的策略构成了直接UPLC-MS证明外星样本中肽序列的第一步。因此，它应该有助于加深对太阳系中可用分子的认识，从而提供有关其化学历史，尤其是地球化学历史的新线索。