The SWI/SNF complex is a highly conserved chromatin remodeling complex and can hydrolyze ATP by its catalytic subunit BRG1 or BRM to reconstruct the chromatin. To investigate whether this ATP-dependent chromatin remodeling could affect the DNA conformation, we therefore regulated (knocked down or overexpressed) BRG1/BRM in the cells and applied Fourier transform infrared (FTIR) spectroscopy to probe DNA conformational changes. As a result, we found that BRG1/BRM was indeed associated with the DNA conformational changes, in which knockdown of BRG1/BRM reduced Z-DNA conformation, while overexpression of BRG1/BRM enhanced Z-DNA conformation. This Z-DNA conformational transformation was also verified using the Z-DNA-binding proteins. Therefore, this work has provided a direct analytical tool to probe Z-DNA transformation upon ATP-dependent chromatin remodeling.

中文翻译：

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重塑染色质诱导通过傅立叶变换红外光谱检测到的Z-DNA构象

SWI / SNF复合物是高度保守的染色质重塑复合物，可通过其催化亚基BRG1或BRM水解ATP以重建染色质。为了研究这种依赖于ATP的染色质重塑是否会影响DNA构象，我们调节了细胞中的BRG1 / BRM（敲低或过表达），并应用了傅里叶变换红外（FTIR）光谱技术来探测DNA构象变化。结果，我们发现BRG1 / BRM确实与DNA构象变化有关，其中敲低BRG1 / BRM会降低Z-DNA构象，而BRG1 / BRM的过表达增强的Z-DNA构象。还使用Z-DNA结合蛋白验证了这种Z-DNA构象转化。因此，这项工作提供了一个直接的分析工具，以探测依赖于ATP的染色质重塑后的Z-DNA转化。