The Journal of Nutritional Biochemistry ( IF 5.6 ) Pub Date : 2020-10-20 , DOI: 10.1016/j.jnutbio.2020.108526 Sarawoot Bunbupha 1 , Poungrat Pakdeechote 2 , Putcharawipa Maneesai 2 , Patoomporn Prasarttong 2
Nobiletin, one of the polymethoxylated flavonoids isolated from citrus peels, is reported to possess various biological activities. The current study investigates the effect and possible mechanisms of nobiletin on nonalcoholic fatty liver disease (NAFLD) in high-fat diet (HFD)-fed rats. Male Sprague-Dawley rats were administrated with HFD and fructose (15%) in drinking water for 16 weeks to induce NAFLD. HFD-fed rats were treated with nobiletin (20 or 40 mg/kg/day) or vehicle for the last 4 weeks. Treatment of HFD-fed rats with nobiletin significantly reduced systolic blood pressure, adiposity, hyperlipidemia, insulin resistance, hepatic lipids content, NAFLD activity score and liver fibrosis. Nobiletin significantly increased plasma adiponectin levels, together with up-regulation of liver adiponectin receptor 1 (AdipoR1) expression. Additionally, decreased malondialdehyde levels and increased superoxide dismutase activity in plasma and hepatic tissue, consistent with down-regulation of liver NADPH oxidase subunit gp91phox expression, were also observed after nobiletin treatment. Furthermore, high dose of nobiletin exhibited higher therapeutic effect as a compared to low dose. These findings suggest that nobiletin alleviates HFD-induced NAFLD and metabolic dysfunction in rats. There might be an association between the observed inhibitory effect of nobiletin on NAFLD and modulation of AdipoR1 and gp91phox.
中文翻译:
川陈皮素通过调节大鼠 AdipoR1 和 gp91phox 表达减轻高脂饮食诱导的非酒精性脂肪肝
川陈皮素是一种从柑橘皮中分离出来的多甲氧基黄酮类化合物,据报道具有多种生物活性。目前的研究调查了川陈皮素对高脂饮食 (HFD) 喂养大鼠的非酒精性脂肪性肝病 (NAFLD) 的影响和可能的机制。雄性 Sprague-Dawley 大鼠在饮用水中服用 HFD 和果糖 (15%) 16 周以诱导 NAFLD。在过去 4 周内,HFD 喂养的大鼠用川陈皮素(20 或 40 毫克/千克/天)或载体治疗。用川陈皮素治疗HFD喂养的大鼠显着降低收缩压、肥胖、高脂血症、胰岛素抵抗、肝脂质含量、NAFLD活性评分和肝纤维化。Nobiletin 显着增加血浆脂联素水平,同时上调肝脂联素受体 1 (AdipoR1) 的表达。此外,phox表达,在川陈皮素处理后也观察到。此外,与低剂量相比,高剂量川川陈皮素表现出更高的治疗效果。这些发现表明川陈皮素可减轻大鼠 HFD 诱导的 NAFLD 和代谢功能障碍。观察到的川陈皮素对 NAFLD 的抑制作用与 AdipoR1 和 gp91 phox 的调节之间可能存在关联。