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Genome-wide characterization and expression profiling of B3 superfamily during flower induction stage in pineapple (Ananas comosus L.)
bioRxiv - Plant Biology Pub Date : 2020-10-19 , DOI: 10.1101/2020.10.19.345181
Cheng Cheng Ruan , Zhe Chen , Fu Chu Hu , Xiang He Wang , Li Jun Guo , Hong Yan Fan , Zhi Wen Luo , Zhi Li Zhang

The B3 superfamily is a plant-specific family, which involves in growth and development process. By now, the identification of B3 superfamily in pineapple ( Ananas comosus ) has not reported. In this study, 57 B3 genes were identified and further phylogenetically classified into five subfamilies, all genes contained B3 domain. Chromosomal localization analysis revealed that 54 of 57 AcB3 genes were located on 21 chromosomes. Collinearity analysis indicated that the segmental duplication was the main event in the evolution of B3 gene superfamily and most of them were under purifying selection. Moreover, there were 7 and 39 pairs of orthologous B3s were identified between pineapple and Arabidopsis or rice, respectively, which indicated the closer genetic relationship between pineapple and rice. Most genes had cis -element of abscisic acid, ethylene, MeJA, light, and abiotic stress. qRT-PCR showed that the expression level of most AcB3 genes were up-regulated within 1 d after ethephon treatment and expressed high level in flower bud differentiation period in stem apex. This study provide a comprehensive understanding of AcB3s and a basis for future molecular studies of ethephon induced pineapple flowering.

中文翻译:

菠萝(Ananas comosus L.)花诱导期B3超家族的全基因组表征和表达谱

B3超家族是植物特有的家族,涉及生长和发育过程。到目前为止,尚未报道菠萝中凤梨中B3超家族的鉴定。在这项研究中,鉴定出57个B3基因,并在系统发育上进一步分为五个亚科,所有基因均包含B3结构域。染色体定位分析显示57个AcB3基因中的54个位于21条染色体上。共线性分析表明,节段重复是B3基因超家族进化的主要事件,其中大部分处于纯化选择中。此外,在菠萝与拟南芥或水稻之间分别鉴定出7对和39对直系同源B3,表明菠萝与水稻之间的亲缘关系更紧密。大多数基因含有脱落酸,乙烯,MeJA,轻度和非生物压力。qRT-PCR显示,乙烯利处理后1 d内,大多数AcB3基因的表达水平上调,茎尖花芽分化期表达量高。这项研究提供了对AcB3s的全面了解,并为将来的乙烯利诱导菠萝开花的分子研究提供了基础。
更新日期:2020-10-20
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