Root-associated bacteria communities are influential to plant growth and stress tolerance. Epigenetic regulation plays important roles in many plant biological processes, but its potential impacts on the assembly of root microbiota remain unclear. Here we report that dysfunction of the histone demethylase IBM1 in Arabidopsis substantially alters root-associated soil bacteria community. We compared two alleles of ibm1 mutant (ibm1-1 and ibm1-4) with wild type Arabidopsis regarding the root-associated bacteria community by 16S rRNA gene sequencing. The constrained principal coordinate analysis (PCoA) showed that the ibm1 mutants are both clearly separated from Col-0 along the major coordinates. Among the 29 families which have a relative abundance more than 0.5% in at least one sample, 10 and 11 families were commonly affected by ibm1-1 and ibm1-4 alleles in the rhizosphere and the endosphere compartment, respectively. Notably, the ACMs (Abundant Community members) belonging Pseudomonadaceae showed increased relative abundance in the ibm1 mutant alleles compared to Col-0 in both the rhizosphere and the endosphere compartments. The ACMs belonging to Oxalobacteraceae mostly showed decreased relative abundance in ibm1 mutants compared to Col-0 in endosphere compartment. These findings demonstrate an influential function of IBM1-mediated epigenetic regulation in shaping the root-associated microbiota.

中文翻译：

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组蛋白脱甲基酶IBM1在拟南芥中的功能障碍重塑了根相关细菌群落

根系相关细菌群落对植物生长和胁迫耐受性有影响。表观遗传调控在许多植物生物学过程中都起着重要作用，但是其对根系菌群装配的潜在影响仍然不清楚。在这里，我们报道在拟南芥中组蛋白脱甲基酶IBM1的功能失调实质上改变了与根相关的土壤细菌群落。我们通过16S rRNA基因测序比较了ibm1突变体的两个等位基因（ibm1-1和ibm1-4）与野生型拟南芥的根相关细菌群落。约束主坐标分析（PCoA）表明，ibm1突变体均沿主要坐标与Col-0清楚地分开。在至少一个样本中相对丰度超过0.5％的29个家庭中，10个和11个家庭通常分别受根际和内球区室的ibm1-1和ibm1-4等位基因影响。值得注意的是，与根际和内球区室的Col-0相比，属于假单胞菌科的ACM（丰富群落成员）在ibm1突变等位基因中显示出相对丰度增加。与内球区室的Col-0相比，属于草酸杆菌科的ACM在ibm1突变体中的相对丰度大多降低。这些发现证明了IBM1介导的表观遗传调控在塑造与根相关的微生物群中的影响功能。属于假单胞菌科的ACM（丰富社区成员）在ibm1突变等位基因中的相对丰度与在根际和内球区室的Col-0相比都增加了。与内球区室的Col-0相比，属于草酸杆菌科的ACM在ibm1突变体中的相对丰度大多降低。这些发现证明了IBM1介导的表观遗传调控在塑造与根相关的微生物群中的影响功能。属于假单胞菌科的ACM（丰富社区成员）在ibm1突变等位基因中的相对丰度与在根际和内球区室的Col-0相比都增加了。与内球区室的Col-0相比，属于草酸杆菌科的ACM在ibm1突变体中的相对丰度大多降低。这些发现证明了IBM1介导的表观遗传调控在塑造与根相关的微生物群中的影响功能。