The autophagy-related genes (ATGs) play important roles in plant growth and response to environmental stresses. Brassica napus (B. napus) is among the most important oilseed crops, but ATGs are largely unknown in this species. Therefore, a genome-wide analysis of the B. napus ATG gene family (BnATGs) was performed. One hundred and twenty-seven ATGs were determined due to the B. napus genome, which belongs to 20 main groups. Segmental duplication occurred more than the tandem duplication in BnATGs. Ka/Ks for the most duplicated pair genes were less than one, which indicated that the negative selection occurred to maintain their function during the evolution of B. napus plants. Based on the results, BnATGs are involved in various developmental processes and respond to biotic and abiotic stresses. One hundred and seven miRNA molecules are involved in the post-transcriptional regulation of 41 BnATGs. In general, 127 simple sequence repeat marker (SSR) loci were also detected in BnATGs. Based on the RNA-seq data, the highest expression in root and silique was related to BnVTI12e, while in shoot and seed, it was BnATG8p. The expression patterns of the most BnATGs were significantly up-regulated or down-regulated responding to dehydration, salinity, abscisic acid, and cold. This research provides information that can detect candidate genes for genetic manipulation in B. napus.

中文翻译：

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不同非生物胁迫和不同组织中油菜（Brassica napus L.）自噬相关基因的计算机模拟分析

自噬相关基因（ATG）在植物生长和对环境胁迫的响应中起重要作用。甘蓝型油菜（B. napus） 是最重要的油料作物之一，但该品种中的ATG很大程度上未知。因此，对甘蓝型油菜ATG基因家族（BnATGs）进行了全基因组分析。由于属于油菜双歧杆菌基因组，共确定了127个ATG ，该基因组属于20个主要组。在BnATG中，分段重复发生的次数多于串联重复发生的次数。重复最多的一对基因的Ka / Ks小于1，这表明负选择的发生是为了在甘蓝型油菜植物的进化过程中保持其功能。根据结果​​，BnATGs参与了各种发育过程，并对生物和非生物胁迫产生反应。一百一十七个miRNA分子参与了41个BnATG的转录后调控。通常，在BnATG中也检测到127个简单序列重复标记（SSR）基因座。基于RNA-seq数据，在根和长角果中的最高表达与BnVTI12e有关，而在芽和种子中的最高表达是BnATG8p。大多数BnATG的表达模式对脱水，盐度，脱落酸和感冒有明显的上调或下调。这项研究提供的信息可以检测甘蓝型油菜的候选基因。