With the rapid progress of nanotechnology, near-infrared (NIR), light-assisted phototherapy as a minimally invasive local cancer therapy, especially photothermal therapy (PTT), has captured broad research attention in recent years. However, combined target molecules with a PTT system through reversible supramolecular interactions has been reported rarely. In this work, we constructed a supramolecular nanosystem combining ATP capture and target PTT based on cationic pillar[5]arene (CWP5)-functionalized Cu_2–xSe nanoparticles (Cu_2–xSe@CWP5 NPs). Cu_2–xSe@CWP5 NPs, with an average diameter of approximately 100 nm and strong absorption in the near-infrared-II window, were prepared in water through a facile one-step in situ synthesis method, then (4-carboxybutyl)triphenylphosphonium bromide (TPP), a mitochondria-targeted molecule, was modified on the surface of the particles through the host–guest recognition. Upon irradiation with a 1064 nm laser, the obtained Cu_2–xSe@CWP5/TPP NPs showed remarkably photothermal ablation capability to HeLa cells. Importantly, our Cu_2–xSe@CWP5/TPP NPs exhibited excellent therapeutic effect due to the combination of inhibited hydrolysis of ATP and targeted photothermal therapy upon in vitro and in vivo studies. Significantly, through host–guest interactions, we can modify different types of target molecules within this PTT system at will

中文翻译：

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阳离子水溶性柱[5]芳烃修饰的 Cu2-xSe 纳米粒子：ATP 的超分子阱及其在 NIR-II 窗口靶向光热治疗中的应用

随着纳米技术的飞速发展，近红外（NIR）光辅助光疗作为一种微创的局部癌症治疗，尤其是光热疗法（PTT），近年来引起了广泛的研究关注。然而，通过可逆超分子相互作用将靶分子与 PTT 系统结合的报道很少。在这项工作中，我们构建了一个基于阳离子柱[5]芳烃（CWP5）功能化的Cu _{2– x} Se 纳米颗粒（Cu _{2– x} Se@CWP5 NPs）结合ATP捕获和靶向PTT的超分子纳米系统。铜_{2– x}Se@CWP5 NPs 平均直径约为 100 nm，在近红外-II 窗口具有强吸收，通过简便的一步原位合成法在水中制备，然后是 (4-羧基丁基) 三苯基溴化鏻 (TPP) )，一种线粒体靶向分子，通过主客体识别在颗粒表面进行修饰。在用 1064 nm 激光照射后，获得的 Cu _{2– x} Se@CWP5/TPP NPs 对 HeLa 细胞显示出显着的光热消融能力。重要的是，我们的 Cu _{2– x}在体外和体内研究中，Se@CWP5/TPP NPs 由于抑制 ATP 水解和靶向光热疗法相结合，表现出优异的治疗效果。重要的是，通过主客体相互作用，我们可以随意修改该 PTT 系统中不同类型的目标分子