Abstract

Background

The GTP exchange factors on ADP-ribosylation factor (ARF) mediate the GDP/GTP exchange on ARF, serve as regulators in protein trafficking and membrane dynamics, and play critical roles in various cell processes. However, the relationship between the expression of ARF-GEF family genes and clinical implications in ovarian cancer remains unclear.

Methods

We performed a systematic investigation on the role of ARF-GEF family genes in ovarian cancer by using Gene Expression Omnibus (GEO), Kaplan-Meier plotter, Gene set enrichment analysis (GSEA), TIMER and TISIDB database.

Results

We found that the ARF-GEF family gene CYTH4 exhibited significant expressional upregulation in ovarian cancer compared to normal ovary tissues. The expression of CYTH4 was also higher in metastases from the omentum than in matched primary ovarian tumours. Kaplan-Meier plotter showed that high expression of CYTH4 predicted worse overall survival, progression free survival and post-progression survival of ovarian cancer patients. Notably, from our correlation analysis, CYTH4 expression showed closely association with tumour-infiltrating immune cells. Intriguingly, the expression of CYTH4 was also significantly correlated with a variety of immunomodulators, chemokines and major histocompatibility complex molecules.

Conclusion

Overall, our findings provide a valuable source of data about the clinical significance of CYTH4 in ovarian cancer.

中文翻译：

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ARF-GEF 家族成员 CYTH4 在卵巢癌中的临床意义和免疫学特征

摘要

背景

ADP-核糖基化因子 (ARF) 上的 GTP 交换因子介导 ARF 上的 GDP/GTP 交换，作为蛋白质运输和膜动力学的调节剂，并在各种细胞过程中发挥关键作用。然而，ARF-GEF 家族基因的表达与卵巢癌临床意义之间的关系仍不清楚。

方法

我们使用基因表达综合 (GEO)、Kaplan-Meier 绘图仪、基因集富集分析 (GSEA)、TIMER 和 TISIDB 数据库对 ARF-GEF 家族基因在卵巢癌中的作用进行了系统研究。

结果

我们发现与正常卵巢组织相比，ARF-GEF 家族基因 CYTH4 在卵巢癌中表现出显着的表达上调。CYTH4 在大网膜转移灶中的表达也高于匹配的原发性卵巢肿瘤。Kaplan-Meier 绘图仪显示 CYTH4 的高表达预测卵巢癌患者的总体生存率、无进展生存率和进展后生存率较差。值得注意的是，从我们的相关分析来看，CYTH4 表达与肿瘤浸润免疫细胞密切相关。有趣的是，CYTH4 的表达也与多种免疫调节剂、趋化因子和主要组织相容性复合物分子显着相关。

结论

总体而言，我们的研究结果提供了有关 CYTH4 在卵巢癌中的临床意义的宝贵数据来源。