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miR-122 regulates the JAK-STAT signalling pathway by down-regulating EPO in the mammary gland during Streptococcus agalactiae-induced mastitis
Italian Journal of Animal Science ( IF 2.5 ) Pub Date : 2020-10-20 , DOI: 10.1080/1828051x.2020.1825996
Junhua Pu 1 , Daijie Chen 1 , Shuangfeng Chu 1 , Zhi Chen 1 , Yongliang Fan 1 , Zhipeng Zhang 1 , Juan J. Loor 2 , Yongjiang Mao 1 , Zhangping Yang 1
Affiliation  

Abstract MicroRNA (miRNA) are single-stranded non-coding RNA of approximately 22 nucleotides estimated to regulate 60% of human and animal genes some of which are associated with cell differentiation, signal transduction and immune processes. Streptococcus agalactiae (S. agalactiae)-induced bovine mastitis is associated with up-regulation of miR-122 and down-regulation of erythropoietin (EPO) abundance in mammary gland tissue. TargetScan analysis revealed that EPO is a predicted target gene of miR-122 and this regulatory relationship was verified by dual-luciferase reporter assay. Overexpression of miR-122 in primary bovine mammary cells led to down-regulation of EPO and also the JAK-STAT signalling pathway genes EPOR, JAK2, STAT5A and STAT5B. Accordingly, the mRNA abundance of BCL-2, a downstream gene in the JAK-STAT signalling pathway was significantly down-regulated. Under conditions of miR-122 inhibition, EPO, EPOR, JAK2, STAT5A and STAT5B were up-regulated. These results demonstrated that, through its action on EPO, miR-122 may play an important role in S. agalactiae-induced mastitis by regulating the JAK-STAT signalling pathway. Highlights Understanding the relationship among miR-122, EPO and the JAK-STAT pathway would be helpful in developing new strategies for controlling mastitis.

中文翻译:

miR-122在无乳链球菌诱导的乳腺炎期间通过下调乳腺中的EPO调节JAK-STAT信号通路

摘要 MicroRNA (miRNA) 是大约 22 个核苷酸的单链非编码 RNA,估计可调节 60% 的人类和动物基因,其中一些基因与细胞分化、信号转导和免疫过程有关。无乳链球菌 (S. agalactiae) 诱导的牛乳腺炎与乳腺组织中 miR-122 的上调和促红细胞生成素 (EPO) 丰度的下调有关。TargetScan 分析显示 EPO 是 miR-122 的预测靶基因,这种调控关系通过双荧光素酶报告基因检测得到验证。miR-122 在原代牛乳腺细胞中的过表达导致 EPO 以及 JAK-STAT 信号通路基因 EPOR、JAK2、STAT5A 和 STAT5B 的下调。因此,BCL-2 的 mRNA 丰度,JAK-STAT 信号通路中的一个下游基因显着下调。在miR-122抑制条件下,EPO、EPOR、JAK2、STAT5A和STAT5B上调。这些结果表明,通过其对 EPO 的作用,miR-122 可能通过调节 JAK-STAT 信号通路在无乳链球菌诱导的乳腺炎中发挥重要作用。重点了解 miR-122、EPO 和 JAK-STAT 通路之间的关系将有助于开发控制乳腺炎的新策略。
更新日期:2020-10-20
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