Antimicrobial resistance (AMR) is a major threat to global health, and it is crucial to understand the epidemiological aspects in order to predict the emergence and propagation of AMR genes. The aim of this study was to assess the variability and medium-term AMR trends within the mostly healthy human population of a single city. We monitored over 36 months (November 2015 to November 2018) the AMR level in the city of Copenhagen, Denmark, by taking bi-weekly sewage samples from the inlets of the three main water treatment plants, extracting the DNA, performing metagenomic sequencing, and read-mapping against a database of known AMR genes. We found that the AMR level was surprisingly stable with no periodic variability and no signs of drift over the measured period. We found, however, that the seemingly random variations at each site correlate in time with each other, suggesting that the variations we see are due to real environmental changes in the occurrence of AMR.

中文翻译：

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哥本哈根污水中的电阻器的长期时间稳定性

抗菌素耐药性（AMR）是对全球健康的主要威胁，因此了解流行病学方面以预测AMR基因的出现和传播至关重要。这项研究的目的是评估单个城市中大多数健康人群的变异性和中期AMR趋势。我们通过从三个主要水处理厂的进水口每两周采集一次污水样本，提取DNA，进行宏基因组测序以及在超过36个月的时间（2015年11月至2018年11月）中监测丹麦哥本哈根的AMR水平。针对已知AMR基因的数据库进行读取映射。我们发现，AMR水平出乎意料地稳定，在测量期间没有周期性变化和漂移迹象。但是，我们发现