In this study, nine experimental diets were formulated to contain three graded levels of protein (40, 45 and 50% of dry diet) and three graded levels of cellulose (0, 5 and 10% of dry diet). These diets were then fed in triplicate to yellow catfish for 90 days. We found that fish fed diets containing 45% and 50% protein had more weight gain than did fish fed a 40% protein diet, while fish fed diets supplemented with 5% cellulose achieved the greatest weight gain and the lower feed conversion ratio. We found that as dietary cellulose and protein levels were increased, the activities of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase), immune response parameters (alkaline phosphatase, acid phosphatase, phagocytic index, respiratory burst, complement 3 and 4) and the mRNA level expression of copper/zinc superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase all rose. In contrast, rising cellulose levels were associated with decreased malondialdehyde contents and increasing tumour necrosis factor, interleukin 1 and interleukin 8 mRNA expression. Overall, our results indicate that dietary protein supplementation can enhance yellow catfish immune functionality and that while dietary protein impacts on the immune response are influenced by dietary cellulose levels, increased dietary cellulose was associated with inflammatory gene upregulation.

中文翻译：

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日粮蛋白质和纤维素水平对少年黄cat鱼（Pelteobagrus fulvidraco）的生长性能，抗氧化状态，免疫反应和炎症的影响

在这项研究中，九种实验饮食被配制为包含三个等级的蛋白质（分别为干饮食的40、45和50％）和三个等级的纤维素（分别为干饮食的0、5和10％）。然后将这些饮食一式三份喂入黄色cat鱼，持续90天。我们发现，饲喂蛋白质含量为45％和50％的鱼的体重增加比饲喂40％蛋白的鱼的体重增加更多，而饲喂添加了5％纤维素的鱼饲料的增重最大，饲料转化率更低。我们发现，随着膳食纤维素和蛋白质水平的提高，抗氧化酶（超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶）的活性，免疫应答参数（碱性磷酸酶，酸性磷酸酶，吞噬指数，呼吸爆发，补体3和4）和mRNA的水平表达铜/锌超氧化物歧化酶，过氧化氢酶，谷胱甘肽过氧化物酶和谷胱甘肽还原酶均上升。相反，升高的纤维素水平与降低的丙二醛含量和增加的肿瘤坏死因子，白介素1和白介素8 mRNA表达有关。总体而言，我们的结果表明，膳食蛋白质补充剂可以增强黄cat鱼的免疫功能，并且尽管膳食蛋白质对免疫反应的影响受膳食纤维素水平的影响，但膳食纤维素的增加与炎症基因的上调相关。