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Biofilm formation onto starch fibres by Bacillus subtilis governs its successful adaptation to chickpea milk
Microbial Biotechnology ( IF 5.7 ) Pub Date : 2020-10-20 , DOI: 10.1111/1751-7915.13665
Satish Kumar Rajasekharan 1 , Tali Paz-Aviram 1 , Shmuel Galili 2 , Zipi Berkovich 3 , Ram Reifen 3 , Moshe Shemesh 1
Affiliation  

Beneficial biofilms may confer effective adaptation to food matrices that assist bacteria in enduring hostile environmental conditions. The matrices, for instance, dietary fibres of various food products, might serve as a natural scaffold for bacterial cells to adhere and grow as biofilms. Here, we report on a unique interaction of Bacillus subtilis cells with the resistant starch fibresof chickpea milk (CPM), herein CPM fibres, along with the production of a reddish-pink pigment. Genetic analysis identified the pigment as pulcherrimin, and also revealed the involvement of Spo0A/SinI pathway in modulating the observed phenotypes. Besides, through successful colonization of the CPM fibres, the wild-type cells of B. subtilis displayed enhanced survivability and resilience to environmental stress, such as heat and in vitro gastrointestinal treatments. In total, we infer that the biofilm formation on CPM fibres is an adaptation response of B. subtilis for strategic survival.

中文翻译:

枯草芽孢杆菌在淀粉纤维上形成的生物膜决定了其对鹰嘴豆奶的成功适应

有益的生物膜可以有效地适应食物基质,帮助细菌在恶劣的环境条件下忍受。例如,各种食品的膳食纤维基质可以作为细菌细胞粘附和生长为生物膜的天然支架。在这里,我们报告了枯草芽孢杆菌细胞与鹰嘴豆奶 (CPM) 的抗性淀粉纤维(此处为 CPM 纤维)的独特相互作用,以及红粉色色素的产生。遗传分析将色素鉴定为普切里明,并且还揭示了 Spo0A/SinI 途径参与调节观察到的表型。此外,通过 CPM 纤维的成功定殖,枯草芽孢杆菌的野生型细胞表现出增强的生存能力和对环境压力的适应能力,例如热和体外胃肠道治疗。总之,我们推断 CPM 纤维上的生物膜形成是枯草芽孢杆菌对战略生存的适应反应。
更新日期:2020-10-20
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