We investigated the feasibility of piglet production by non‐surgical embryo transfer (Ns‐ET) of vitrified porcine blastocysts and expanded blastocysts transported to commercial farms and warmed on site (V/T/W embryos). Ns‐ET was performed by depositing 11–20 vitrified and warmed embryos at a proximal site within the uterus via a catheter. In Experiment 1, the effect of donor–recipient estrous cycle asynchrony on the efficiency of Ns‐ET of vitrified and ordinary warmed embryos was investigated at the experimental facility. With a 1‐day delay recipients relative to that of donor, the farrowing rate was 50.0% and the survival rate to term was 21.1%. In Experiment 2, Ns‐ET using recipients with a 1‐day delay and vitrified embryos after one‐step warming and dilution was evaluated at the experimental facility. Although the resulting farrowing rate was 42.9%, the survival rate was 6.4%. In Experiment 3, Ns‐ET was conducted using V/T/W embryos at four commercial farms, where piglets derived from them were produced. When artificial insemination was conducted prior to Ns‐ET, the farrowing and survival rates obtained using V/T/W embryos were 75.0%, and 21.3%, respectively. These results show that Ns‐ET of V/T/W embryos using this protocol would be feasible for piglet production at farms.

中文翻译：

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通过玻璃化胚胎的非手术移植生产小猪，运送到商业养猪场并在现场加热

我们调查了通过非手术胚胎移植 (Ns-ET) 将玻璃化猪胚泡和扩大的胚泡运送到商业农场并在现场加热（V/T/W 胚胎）生产仔猪的可行性。Ns-ET 是通过导管在子宫内的近端放置 11-20 个玻璃化和加热的胚胎来进行的。在实验 1 中，在实验设施中研究了供体-受体动情周期不同步对玻璃化和普通加热胚胎的 Ns-ET 效率的影响。与捐赠者相比，接受者延迟 1 天，分娩率为 50.0%，足月存活率为 21.1%。在实验 2 中，Ns-ET 使用延迟 1 天的受体和经过一步升温和稀释后的玻璃化胚胎，在实验设施中进行了评估。虽然由此产生的分娩率为 42.9%，但存活率为 6.4%。在实验 3 中，在四个商业农场使用 V/T/W 胚胎进行了 Ns-ET，并生产了来自它们的仔猪。当在 Ns-ET 之前进行人工授精时，使用 V/T/W 胚胎获得的分娩率和存活率分别为 75.0% 和 21.3%。这些结果表明，使用该方案的 V/T/W 胚胎的 Ns-ET 对于农场的仔猪生产是可行的。