Herein we report a reliable and stable biosensor for the amperometric detection of creatinine through the ammonium produced in its reaction with creatinine deiminase linked to a Nafion®/polyaniline gold coated surface. The gold electrode array was produced using a low cost printed board circuit technology. A linear range of 1–1000 μM was obtained with standard deviations of less than 5 % (n=3) obtained. Stability studies were carried out and 80 % activity was maintained after one month. The sensors were tested with 142 serum samples from patients with renal dysfunction at different stage of the disease and validated using a commercial colorimetric method.

中文翻译：

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基于印刷电路板技术制造的金电极阵列的临床样品中肌酐的安培检测

在此，我们报告了一种可靠而稳定的生物传感器，用于通过安培检测，该安培检测是通过与与Nafion®/聚苯胺金包被的表面连接的肌酐脱亚氨酶反应生成的铵进行的。金电极阵列是使用低成本印刷电路板技术生产的。线性范围为1–1000μM，标准偏差小于5％（n = 3）。进行了稳定性研究，一个月后保持80％的活性。用来自疾病不同阶段的肾功能不全患者的142份血清样品对传感器进行了测试，并使用商业比色法进行了验证。