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Increased Expression of the Multimerin-1 Gene in α-Synuclein Knokout Mice
Doklady Biological Sciences Pub Date : 2020-10-20 , DOI: 10.1134/s0012496620050014
K D Chaprov 1, 2 , V V Goloborshcheva 2, 3 , T V Tarasova 1, 2 , E V Teterina 1 , M V Korokin 4 , V O Soldatov 4 , M V Pokrovskiy 4 , V G Kucheryanu 3 , S G Morozov 3 , R K Ovchinnikov 1
Affiliation  

Abstract

Multimerin-1 (Mmrn-1) is a soluble protein, also known as elastin microfibril interfacer 4 (EMILIN-4), found in platelets and in the endothelium of blood vessels. Its function and role in pathology are still not fully understood. Genetic modifications in alpha-synuclein gene (Snca) locus that mapped 160 Kb apart from Mmrn-1 in mouse genome, could weigh with regulatory elements of Mmrn-1 gene. We have studied the Mmrn-1 expression in brain cortex of three mouse lines with Snca knock-out: B6(Cg)-Sncatm1.2Vlb/J, B6;129-Sncatm1Sud/J, and B6;129X1-Sncatm1Rosl/J. The 35-fold increase for Mmrn-1 mRNA level have been found in B6;129X1-Sncatm1Rosl/J mice that carry in their genome foreign sequences including bacterial gene neo and a strong promoter of a mouse phosphoglycerate kinase (Pgk1) oriented towards Mmrn-1 gene. This effect on regulatory elements of Mmrn-1 gene as a result of modifications in Snca locus should be taken into consideration when using B6;129X1-Sncatm1Rosl/J line, that is widely applied for study of neurodegeneration mechanisms.



中文翻译:

Multimerin-1基因在α-突触核蛋白基因敲除小鼠中的表达增加

摘要

Multimerin-1(Mmrn-1)是一种可溶性蛋白,也称为弹性蛋白微纤维接口4(EMILIN-4),存在于血小板和血管内皮中。其在病理中的功能和作用仍未完全了解。小鼠基因组中与Mmrn-1距离160 Kb的α-突触核蛋白基因(Snca)基因座的遗传修饰可能与Mmrn-1基因的调控元件有关。我们研究了三只Snca基因敲除小鼠系在大脑皮层中Mmrn-1的表达:B6(Cg)-Snca tm1.2Vlb / J,B6; 129-Snca tm1Sud / J,和B6; 129X1-Snca tm1Rosl / J. 在B6; 129X1-Snca tm1Rosl中发现Mmrn-1 mRNA水平增加了35倍/ J小鼠在其基因组中携带外源序列,包括细菌基因neo和面向Mmrn-1基因的小鼠磷酸甘油酸激酶(Pgk1)的强启动子。当使用B6; 129X1-Snca tm1Rosl / J系时,应考虑由于Snca基因座的修饰而对Mmrn-1基因的调控元件产生的影响,该系已广泛用于研究神经退行性机制。

更新日期:2020-10-20
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