Marine mammal cell cultures are a multifunctional instrument for acquiring knowledge about life in the world’s oceans in physiological, biochemical, genetic, and ecotoxicological aspects. We succeeded in isolation, cultivation, and characterization of skin fibroblast cultures from five marine mammal species. The cells of the spotted seal (Phoca largha), the sea lion (Eumetopias jubatus), and the walrus (Odobenus rosmarus) are unpretentious to the isolation procedure. The sea otter (Enhydra lutris) fibroblasts should be isolated by trypsin disaggregation, while only mechanical disaggregation was suitable for the beluga whale (Delphinapterus leucas) cells. The cell growth parameters have been determined allowing us to find the optimal seeding density for continuous and effective cultivation. The effects of nonpathogenic algal extracts on proliferation, viability, and functional activity of marine mammal cells in vitro have been presented and discussed for the first time.

海洋哺乳动物细胞培养是一种多功能工具，可用于从生理、生化、遗传和生态毒理学方面获取有关世界海洋生命的知识。我们成功地从五种海洋哺乳动物物种中分离、培养和表征了皮肤成纤维细胞培养物。斑海豹 ( Phoca largha )、海狮 ( Eumetopias jubatus ) 和海象 ( Odobenus rosmarus ) 的细胞在隔离过程中并不做作。海獭（Enhydra lutris）成纤维细胞应通过胰蛋白酶解聚来分离，而白鲸（Delphinapterus leucas）仅适用于机械解聚。） 细胞。已确定细胞生长参数，使我们能够找到持续有效培养的最佳接种密度。首次提出和讨论了非致病性藻类提取物对体外海洋哺乳动物细胞增殖、活力和功能活性的影响。