In 2012, we provided the first published evidence that human pluripotent stem cells could be differentiated to cells exhibiting markers and phenotypes characteristic of the blood–brain barrier (BBB). In the ensuing years, the initial protocols have been refined, and the research community has identified both positive and negative attributes of this stem cell-based BBB model system. Here, we give our perspective on the current status of these models and their use in the BBB community, as well as highlight key attributes that would benefit from improvement moving forward.

中文翻译：

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基于人类多能干细胞的血脑屏障模型评述

2012 年，我们提供了第一个已发表的证据，表明人类多能干细胞可以分化为具有血脑屏障 (BBB) 特征标志物和表型的细胞。在随后的几年里，最初的协议得到了改进，研究界已经确定了这种基于干细胞的 BBB 模型系统的积极和消极属性。在这里，我们对这些模型的当前状态及其在 BBB 社区中的使用给出了自己的看法，并重点介绍了将从改进中受益的关键属性。