For an effective medical application of therapeutic siRNA, a safe and an efficient delivery system are required. Herein, magnetic nanoparticles (MNPs) have been successfully used as siRNA delivery vehicles. Firstly, MNPs were coated with gold (Au) nanoparticles and then capped with PEI. To improve the biocompatibility of nanoparticles, hyaluronic acid (HA) was coated onto the surface of PEI-Au/Fe nanoparticles. The prepared HA-PEI-Au/Fe3O4 nanoparticles were characterized and found to be uniform and well segregated in TEM analysis. FTIR analysis confirmed that HA was successfully conjugated to PEI. The polymer content in these nanoparticles was relatively higher than PEG coated nanoparticles. Cell viability assay demonstrated that the nanoparticles were relatively biocompatible in nature. ADAM10 siRNA was loaded into the HA-PEI-Au/Fe3O4 nanoparticles and cytotoxicity to prostate cancer (PC3) cells was analyzed. The results indicate that ADAM10 siRNA loaded HA-PEI-Au/Fe3O4 suppress the PC3 cells growth in vitro. Clearly, it could be confirmed that HA-PEI coated Au/Fe3O4 nanoparticles with higher biocompatibility appear to be suitable for intracellular siRNA delivery.

中文翻译：

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聚乙烯亚胺包覆的金磁性纳米粒子用于在前列腺癌细胞中递送 ADAM10 siRNA

对于治疗性 siRNA 的有效医学应用，需要安全有效的递送系统。在此，磁性纳米粒子 (MNP) 已成功用作 siRNA 递送载体。首先，MNPs 涂有金 (Au) 纳米颗粒，然后用 PEI 覆盖。为了提高纳米粒子的生物相容性，将透明质酸 (HA) 涂覆在 PEI-Au/Fe 纳米粒子的表面上。对制备的 HA-PEI-Au/Fe3O4 纳米粒子进行表征，发现其在 TEM 分析中均匀且分离良好。FTIR 分析证实 HA 成功偶联到 PEI。这些纳米颗粒中的聚合物含量相对高于 PEG 包覆的纳米颗粒。细胞活力测定表明纳米颗粒在性质上具有相对的生物相容性。ADAM10 siRNA 被加载到 HA-PEI-Au/Fe3O4 纳米颗粒中，并分析了对前列腺癌 (PC3) 细胞的细胞毒性。结果表明，ADAM10 siRNA 负载的 HA-PEI-Au/Fe3O4 在体外抑制 PC3 细胞的生长。显然，可以证实具有更高生物相容性的 HA-PEI 涂覆的 Au/Fe3O4 纳米粒子似乎适合细胞内 siRNA 递送。