Human extended pluripotent stem cells (EPSCs), with bidirectional chimeric ability to contribute to both embryonic and extra-embryonic lineages, can be obtained and maintained by converting embryonic stem cells (ESCs) using chemicals. However, the transition system is based on inactivated mouse fibroblast, which greatly hinders the mechanistic studies of extended pluripotency and further applications. Here we reported a Matrigel-based feeder-free method to convert human ESCs and iPSCs into EPSCs and demonstrated the extended pluripotency in terms of molecular features, chimeric ability, and transcriptome. We further improved the protocol by applying chemicals targeting glycolysis and histone methyltransferase. Altogether, our data established a feeder-free system to generate human EPSCs and provided additional insights into the acquisition of extended pluripotency.

中文翻译：

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无饲养层条件下人扩展多能干细胞的衍生

通过使用化学物质转换胚胎干细胞（ESC），可以获得并维持具有双向嵌合能力以同时促进胚胎和胚外谱系的人扩展多能干细胞（EPSC）。但是，过渡系统基于灭活的小鼠成纤维细胞，这极大地阻碍了扩展多能性和进一步应用的机理研究。在这里，我们报道了一种基于Matrigel的无饲养者方法，将人ESC和iPSC转换为EPSC，并展示了在分子特征，嵌合能力和转录组方面的扩展多能性。我们通过应用针对糖酵解和组蛋白甲基转移酶的化学品进一步改善了方案。共，