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Overexpression of CDSP32 (GhTRX134) Cotton Gene Enhances Drought, Salt, and Oxidative Stress Tolerance in Arabidopsis
Plants ( IF 4.658 ) Pub Date : 2020-10-19 , DOI: 10.3390/plants9101388
Mohammed Elasad , Adeel Ahmad , Hantao Wang , Liang Ma , Shuxun Yu , Hengling Wei

Upland cotton (Gossypium hirsutum L.) is the main natural fiber crop worldwide and is an essential source of seed oil and biofuel products. Many abiotic stresses, such as drought and salinity, constrain cotton production. Thioredoxins (TRXs) are a group of small ubiquitous proteins that are widely distributed among organisms. TRXs play a crucial role in regulating diverse functions during plant growth and development. In the present study, a novel GhTRX134 gene was characterized and overexpressed in Arabidopsis and silenced in cotton under drought stress. Furthermore, the proline content and enzyme activity levels were measured in transgenic plants and wild-type (Wt) plants under drought and salt stress. The results revealed that the overexpression of GhTRX134 enhanced abiotic stress tolerance. When GhTRX134 was silenced, cotton plants become more sensitive to drought. Taken together, these findings confirmed that the overexpression of GhTRX134 improved drought and salt tolerance in Arabidopsis plants. Therefore, the GhTRX134 gene can be transformed into cotton plants to obtain transgenic lines for more functional details.

中文翻译:

CDSP32(GhTRX134)棉花基因的过表达增强拟南芥的干旱,盐分和氧化胁迫耐受性

陆地棉(Gossypium hirsutum L.)是全球主要的天然纤维作物,也是种子油和生物燃料产品的重要来源。许多非生物胁迫(例如干旱和盐碱化)限制了棉花的产量。硫氧还蛋白(TRXs)是一组在生物体中广泛分布的小型泛在蛋白。TRX在调节植物生长发育过程中的各种功能中起着至关重要的作用。在本研究中,一个新的GhTRX134基因在拟南芥中过表达并在干旱胁迫下在棉花中沉默。此外,在干旱和盐胁迫下,在转基因植物和野生型(Wt)植物中测量了脯氨酸含量和酶活性水平。结果表明,过表达GhTRX134增强了非生物胁迫耐受性。当GhTRX134静音时,棉株对干旱变得更加敏感。综上所述,这些发现证实了GhTRX134的过表达改善了拟南芥植物的干旱和耐盐性。因此,可以将GhTRX134基因转化到棉花植物中以获得更多功能细节的转基因品系。
更新日期:2020-10-19
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