当前位置: X-MOL 学术Int. J. Fruit Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Induction of Polyploidy in C35 Citrange through in Vitro Colchicine Treatments of Seed-Derived Explants
International Journal of Fruit Science ( IF 2.4 ) Pub Date : 2020-09-21 , DOI: 10.1080/15538362.2020.1837051
Berken Cimen 1
Affiliation  

ABSTRACT Polyploidy can be observed in citrus species which often enables better adaptation to environmental stresses. In this context, this paper aimed to develop stable tetraploid C35 citrange by in vitro colchicine treatments. Seed-derived explants were obtained by a germination experiment using several concentrations of GA3. Culturing seeds of C35 without seed coat on MT basal medium with the addition of 4 mg l−1 GA3 resulted in the highest germination rate (84%). Various colchicine concentrations and exposure time were tested to induce polyploidization. Seed-derived explants of C35 citrange treated with 0.1% colchicine for 48 h demonstrated high rates of mutation for polyploidization and the highest tetraploid induction percentage (15%) was determined in this treatment. Higher colchicine concentrations and exposure time decreased the survival rates of the seedlings. Flow cytometry demonstrated that the nuclear genome size of tetraploids (1.598 pg/2 C) was significantly (P < .01) higher than diploid (0.794 pg/2 C) C35 seedlings. Recovered seedlings were identified with morphological and cytological variables, such as leaf area and stomata size. Generally, leaf area and stomata size significantly increased with the increasing ploidy level. The method used in this present study was relevant and an effective way to induce polyploidy in C35 citrange.

中文翻译:

通过种子衍生外植体的体外秋水仙碱处理诱导 C35 柑橘多倍体

摘要 在柑橘物种中可以观察到多倍性,这通常能够更好地适应环境压力。在此背景下,本文旨在通过体外秋水仙碱处理开发稳定的四倍体 C35 柑橘。种子来源的外植体是通过使用几种浓度的 GA3 的发芽实验获得的。在添加 4 mg l-1 GA3 的 MT 基础培养基上培养没有种皮的 C35 种子导致最高的发芽率 (84%)。测试了各种秋水仙碱浓度和暴露时间以诱导多倍体化。用 0.1% 秋水仙碱处理 48 小时的 C35 柑橘种子外植体显示出高多倍化突变率,并且在该处理中确定了最高的四倍体诱导百分比 (15%)。较高的秋水仙碱浓度和暴露时间会降低幼苗的存活率。流式细胞术表明,四倍体 (1.598 pg/2 C) 的核基因组大小显着 (P < .01) 高于二倍体 (0.794 pg/2 C) C35 幼苗。通过形态学和细胞学变量(例如叶面积和气孔大小)对恢复的幼苗进行鉴定。一般来说,叶面积和气孔大小随着倍性水平的增加而显着增加。本研究中使用的方法是在 C35 柑橘中诱导多倍体的相关且有效的方法。回收的幼苗通过形态学和细胞学变量(例如叶面积和气孔大小)进行鉴定。一般来说,叶面积和气孔大小随着倍性水平的增加而显着增加。本研究中使用的方法是在 C35 柑橘中诱导多倍体的相关且有效的方法。回收的幼苗通过形态学和细胞学变量(例如叶面积和气孔大小)进行鉴定。一般来说,叶面积和气孔大小随着倍性水平的增加而显着增加。本研究中使用的方法是在 C35 柑橘中诱导多倍体的相关且有效的方法。
更新日期:2020-09-21
down
wechat
bug