Abstract ContextQing-Mai-Yin (QMY) is a clinically used herbal formula for treating arteriosclerosis obliterans (ASO). Objective To evaluate the chemical constituents and effects of QMY on ASO rabbit model. Materials and methods Forty-eight New Zealand rabbits were divided into six groups (n = 8): normal (normal rabbits treated with 0.5% CMC-Na), vehicle (ASO rabbits treated with 0.5% CMC-Na), positive (simvastatin, 1.53 mg/kg), and QMY treatment (300, 600, and 1200 mg/kg). ASO rabbit model was prepared by high fatty feeding, roundly shortening artery, and bovine serum albumin immune injury. QMY (300, 600 and 1200 mg/kg) was orally administered for 8 weeks. The effects and possible mechanisms of QMY on ASO rabbits were evaluated by pathological examination, biochemical assays, and immunohistochemical assays. The compositions of QMY were analysed using HPLC-Q-TOF-MS/MS analysis. Results Compared to the vehicle rabbit, QMY treatment suppressed plaque formation and intima thickness in aorta, and decreased intima thickness, whereas increased lumen area of femoral artery. Additionally, QMY treatment decreased TC, TG and LDL, decreased CRP and ET, and increased NO and 6-K-PGF1α in serum. Furthermore, the potential mechanisms studied revealed that QMY treatment could suppress expression of TNF-α, IL-6, ICAM-1 and NF-κB in endothelial tissues, and increase IκB. In addition, HPLC analysis showed QMY had abundant anthraquinones, stilbenes, and flavonoids. Conclusion QMY has ameliorative effects on ASO rabbit, and the potential mechanisms are correlated to reducing inflammation and down-regulating NF-κB. Our study provides a scientific basis for the future application and investigation of QMY.

中文翻译：

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中药清脉饮对兔动脉硬化闭塞症的改善作用

摘要 背景清脉饮（QMY）是临床上用于治疗动脉硬化闭塞症（ASO）的中草药方。目的评价QMY的化学成分及其对ASO兔模型的影响。材料和方法 将 48 只新西兰兔分为六组（n = 8）：正常（用 0.5% CMC-Na 处理的正常兔）、载体（用 0.5% CMC-Na 处理的 ASO 兔）、阳性（辛伐他汀、 1.53 毫克/千克）和 QMY 处理（300、600 和 1200 毫克/千克）。ASO兔模型采用高脂肪喂养、全面缩短动脉、牛血清白蛋白免疫损伤法制备。QMY（300、600 和 1200 mg/kg）口服给药 8 周。通过病理学检查、生化试验和免疫组化试验评估QMY对ASO兔的影响和可能的机制。使用 HPLC-Q-TOF-MS/MS 分析法分析 QMY 的组成。结果与载体兔相比，QMY 治疗抑制了主动脉中的斑块形成和内膜厚度，并降低了内膜厚度，而增加了股动脉管腔面积。此外，QMY 治疗降低了 TC、TG 和 LDL，降低了 CRP 和 ET，并增加了血清中的 NO 和 6-K-PGF1α。此外，研究的潜在机制表明，QMY 治疗可以抑制内皮组织中 TNF-α、IL-6、ICAM-1 和 NF-κB 的表达，并增加 IκB。此外，HPLC 分析表明 QMY 含有丰富的蒽醌、芪和黄酮类化合物。结论 QMY对ASO兔具有改善作用，其潜在机制与减轻炎症和下调NF-κB有关。