ABSTRACT

Background: Intake of probiotic bacteria may prevent oral Candida infection.

Objective: To screen the antifungal activity of 14 Lactobacillus candidate strains of human origin, against six opportunistic C. albicans and non-albicans species. A second aim was to study the acid production of the four strains showing the strongest antifungal activity.

Methods: We used an agar overlay growth inhibition assay to the assess the antifungal activity of the lactobacilli. The acid-producing capacity was measured with pH micro-sensors.

Results: All 14 Lactobacillus candidates inhibited the growth of the Candida spp. The four best-performing strains were L. rhamnosus DSM 32992 (oral origin), L. rhamnosus DSM 32991 (oral), L. jensenii 22B42 (vaginal), and L. rhamnosus PB01 (vaginal). The difference between L. rhamnosus DSM 32992 and the other three strains was statistically significant (p < 0.001). The Candida spp. differed in susceptibility; C. parapsilosis was highly inhibited, while C. krusei was not or slightly inhibited. The oral L. rhamnosus DSM 32992 and DSM 32991 strains showed the lowest pH-values.

Conclusion: Screening of probiotic lactobacilli showed significant strain-dependent variations in their antifungal capacity in a pH-dependent mode. Two strains of oral origin were most effective. A further characterization seems justified to elaborate on their probiotic properties.

摘要

背景：摄入益生菌可能会预防口腔 念珠菌 感染。

目的：筛选14 种人源乳酸杆菌菌株对6种机会性 白色念珠菌 和非白色念珠菌的抗真菌活性 。第二个目的是研究显示出最强抗真菌活性的四种菌株的酸产生。

方法：我们使用琼脂覆盖生长抑制试验来评估乳酸菌的抗真菌活性。用pH微型传感器测量产酸能力。

结果：所有14种  候选乳酸杆菌均抑制念珠菌的生长  。四个表现最佳的菌株是 鼠李糖乳杆菌 DSM 32992（口服）， 鼠李糖乳杆菌 DSM 32991（口服）， 詹 氏乳杆菌22B42（阴道）和 鼠李糖乳杆菌 PB01（阴道）。鼠李糖乳杆菌 DSM 32992和其他三个菌株之间的差异 具有统计学意义（p <0.001）。该 念珠菌 属。敏感性不同； C. parapsilosis 被高度抑制，而 krusei C. 没有或被轻微抑制。口服 鼠李糖乳杆菌 DSM 32992和DSM 32991菌株显示最低的pH值。

结论：益生菌乳酸菌的筛选在pH依赖性模式下显示出显着的菌株依赖性抗真菌能力变化。两种口服来源的菌株最有效。进一步鉴定其益生菌特性似乎是合理的。