This study evaluated the gene expression profile of the human adipose-derived stem cells (hASCs) grown on the Biosilicate^®/F18 glass (BioS-2P/F18) scaffolds. hASCs were cultured using the osteogenic medium (control), the scaffolds, and their ionic extract. We observed that ALP activity was higher in hASCs grown on the BioS-2P/F18 scaffolds than in hASCs cultured with the ionic extract or the osteogenic medium on day 14. Moreover, the dissolution product group and the control exhibited deposited calcium, which peaked on day 21. Gene expression profiles of cell cultured using the BioS-2P/F18 scaffolds and their extract were evaluated in vitro using the RT² Profiler polymerase chain reaction (PCR) microarray on day 21. Mineralizing tissue-associated proteins, differentiation factors, and extracellular matrix enzyme expressions were measured using quantitative PCR. The gene expression of different proteins involved in osteoblast differentiation was significantly up-regulated in hASCs grown on the scaffolds, especially BMP1, BMP2, SPP1, BMPR1B, ITGA1, ITGA2, ITGB1, SMAD1, and SMAD2, showing that both the composition and topographic features of the biomaterial could stimulate osteogenesis. This study demonstrated that gene expression of hASCs grown on the scaffold surface showed significantly increased gene expression related to hASCs cultured with the ionic extract or the osteogenic medium, evidencing that the BioS-2P/F18 scaffolds have a substantial effect on cellular behavior of hASCs.

中文翻译：

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生物活性生物硅酸盐®/F18玻璃支架对人脂肪干细胞成骨分化的影响

^{本研究评估了在 Biosilicate ®} /F18 玻璃 (BioS-2P/F18) 支架上生长的人类脂肪干细胞 (hASC) 的基因表达谱。使用成骨培养基（对照）、支架及其离子提取物培养 hASC。我们观察到，在第 14 天，在 BioS-2P/F18 支架上生长的 hASCs 中的 ALP 活性高于用离子提取物或成骨培养基培养的 hASCs。此外，溶出产物组和对照组表现出沉积的钙，在第 21 天。使用 BioS-2P/F18 支架培养的细胞及其提取物的基因表达谱在体外使用 RT ^{2进行了评估}第 21 天的 Profiler 聚合酶链反应 (PCR) 微阵列。使用定量 PCR 测量矿化组织相关蛋白、分化因子和细胞外基质酶的表达。在支架上生长的 hASCs 中，参与成骨细胞分化的不同蛋白质的基因表达显着上调，尤其是 BMP1、BMP2、SPP1、BMPR1B、ITGA1、ITGA2、ITGB1、SMAD1 和 SMAD2，表明组成和形貌特征均显着上调。的生物材料可以刺激成骨。该研究表明，在支架表面生长的 hASCs 的基因表达显示出与用离子提取物或成骨培养基培养的 hASCs 相关的基因表达显着增加，证明 BioS-2P/F18 支架对 hASCs 的细胞行为具有显着影响。