用于超低浓度 DNA 检测的无标记近红外等离子体传感技术,Advanced Science

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用于超低浓度 DNA 检测的无标记近红外等离子体传感技术
Advanced Science ( IF 15.1 ) Pub Date : 2020-10-19 , DOI: 10.1002/advs.202000763
Shimeng Chen ₁ , Chuan Liu ₂ , Yun Liu ₃ , Qiang Liu ₃ , Mengdi Lu ₃ , Sheng Bi ₄ , Zhenguo Jing ₃ , Qingxu Yu ₁ , Wei Peng ₃

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低浓度的生物分子检测通常是生物测量和早期疾病诊断的最重要标准。在本文中，通过在连续镀金纳米三角形阵列上实现近红外等离子体激发，展示了一种高度灵敏的纳米等离子体生物传感方法。与传统配置相比，小入射角的近红外入射光由于其更大的相互作用体积和更强的电场强度而激发表面等离子体共振，具有更高的光谱灵敏度。通过引入尖锐的纳米三角形金属尖端，实现了等离子体近场的强烈局域化，从而增强了传感表面的分子感知能力。这种方法具有增强的灵敏度（42103.8 nm/RIU）和高品质因数（367.812），实现了纳摩尔水平的 ssDNA 直接测定，这是无标记超灵敏传感技术的又一步。基于纳米三角形和金纳米粒子之间的耦合效应，单链DNA的检测限显着提高为1.2 × 10 ^-18 m 。这项工作结合了高体积和表面敏感性，为无标记超低浓度生物分子检测提供了一种简单的方法。

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Label‐Free Near‐Infrared Plasmonic Sensing Technique for DNA Detection at Ultralow Concentrations

Biomolecular detection at a low concentration is usually the most important criterion for biological measurement and early stage disease diagnosis. In this paper, a highly sensitive nanoplasmonic biosensing approach is demonstrated by achieving near‐infrared plasmonic excitation on a continuous gold‐coated nanotriangular array. Near‐infrared incident light at a small incident angle excites surface plasmon resonance with much higher spectral sensitivity compared with traditional configuration, due to its greater interactive volume and the stronger electric field intensity. By introducing sharp nanotriangular metallic tips, intense localization of plasmonic near‐fields is realized to enhance the molecular perception ability on sensing surface. This approach with an enhanced sensitivity (42103.8 nm per RIU) and a high figure of merit (367.812) achieves a direct assay of ssDNA at nanomolar level, which is a further step in label‐free ultrasensitive sensing technique. Considerable improvement is recorded in the detection limit of ssDNA as 1.2 × 10⁻¹⁸ m based on the coupling effect between nanotriangles and gold nanoparticles. This work combines high bulk‐ and surface‐sensitivities, providing a simple way toward label‐free ultralow‐concentration biomolecular detection.

更新日期：2020-12-03

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