The current study was concentrated to detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells. The partial purification of TLC was moved three different active spots. Among the spots, the third spot of the extract was shown excellent anti-cancer properties against MCF-7 human breast cancer cells. The available anti-cancer compounds present in the partially purified extract was confirmed by GC–MS data interpretation. Three fractions of the compounds were purified by preparative HPLC and their respective spectrum was confirmed by analytical HPLC. Active anti-cancer fraction was separately purified by preparative HPLC and the cytotoxicity result was shown with 100 µg/mL concentration. The anti-cancer compound of pyrrolo [1,2-a]pyrazine-1,4-dione, hexahydro-3 was detected by LC-MS analysis after interpretation with GC–MS data. Further, the morphological damages and increased proliferation with more death cells of pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3 treated MCF-7 were observed by phase contrast and fluorescence microscope techniques. Finally, the nuclear condensation and increased necrotic cells of the pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3 treated MCF-7 cells was clearly identified by fluorescence microscope techniques using Hoechst 33,342 stain. Therefore, our result was prove, the pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3 rich Streptomyces akiyoshiensis GRG 6 (KY457710) as a potential anti-cancer agent against MCF-7 breast cancer cells.

目前的研究集中于从海洋吉吉链霉菌中检测抗癌化合物针对MCF-7乳腺癌细胞的GRG 6（KY457710）。将TLC的部分纯化移至三个不同的活性点。在斑点中，提取物的第三斑点显示出对MCF-7人乳腺癌细胞的优异的抗癌特性。GC-MS数据解释证实了部分纯化的提取物中存在的可用抗癌化合物。通过制备型HPLC纯化三部分化合物，并通过分析型HPLC确认其各自的光谱。活性抗癌组分分别通过制备型HPLC纯化，并以100 µg / mL的浓度显示细胞毒性结果。用GC-MS数据解释后，通过LC-MS分析检测到吡咯并[1,2-a]吡嗪-1,4-二酮，六氢-3的抗癌化合物。进一步，通过相差和荧光显微镜技术观察到吡咯并[1,2-a]吡嗪-1,4-二酮，六氢-3处理的MCF-7具有更多的死亡细胞的形态学损伤和增殖增加。最后，吡咯并[1,2-a]吡嗪-1,4-二酮，六氢-3处理的MCF-7细胞的核浓缩和坏死细胞的增加已通过使用Hoechst 33,342染料的荧光显微镜技术进行了清晰鉴定。因此，我们的结果证明，吡咯并[1,2-a]吡嗪-1,4-二酮，六氢-3 通过荧光显微镜技术使用Hoechst 33,342染色剂清楚地识别了六氢3处理的MCF-7细胞。因此，我们的结果证明，吡咯并[1,2-a]吡嗪-1,4-二酮，六氢-3 通过荧光显微镜技术使用Hoechst 33,342染色剂清楚地识别了六氢3处理的MCF-7细胞。因此，我们的结果证明，吡咯并[1,2-a]吡嗪-1,4-二酮，六氢-3akiyoshiensis GRG 6链霉菌（KY457710）作为针对MCF-7乳腺癌细胞的潜在抗癌药。