Outer membrane vesicles (OMVs) of Neisseria meningitidis contain important antigens to trigger an immune response against meningococci and have been studied as vaccines compounds. The immune response to a vaccine may be affected by its constitution and route of administration. Therefore, Swiss mice were immunized by different routes with OMVs of N. meningitidis B with dimethyl dioctadecyl ammonium bromide in bilayer fragments (DDA-BF) or aluminum hydroxide (AH) as adjuvants. The adjuvants and different routes were compared regarding the immune responses by ELISA, western blot, delayed type hypersensitivity (DTH) and histopathologic analysis. The antigenic preparation generated humoral and cellular immune responses. In quantitative analyzes, in general, AH was superior to DDA-BF. However, analysis such as IgG avidity index, bactericidal activity and immunoblot, revealed no important differences regarding the adjuvant or route of immunization. Regarding the parameters tested, it was not possible to define a superiority between the adjuvants and routes of immunization proposed by this study.

脑膜炎奈瑟氏球菌的外膜囊泡（OMV）包含重要的抗原，可触发针对脑膜炎球菌的免疫反应，并已作为疫苗化合物进行了研究。对疫苗的免疫反应可能受其组成和给药途径的影响。因此，瑞士小鼠通过脑膜炎奈瑟氏菌OMV的不同途径免疫B以二甲基双十八烷基溴化铵的双层片段（DDA-BF）或氢氧化铝（AH）作为佐剂。通过ELISA，免疫印迹，迟发型超敏反应（DTH）和组织病理学分析比较了佐剂和不同途径的免疫应答。抗原制剂产生体液和细胞免疫应答。通常，在定量分析中，AH优于DDA-BF。但是，诸如IgG亲和力指数，杀菌活性和免疫印迹等分析表明，在佐剂或免疫途径方面没有重要差异。关于测试的参数，不可能在佐剂和本研究提出的免疫途径之间定义优越性。