Abstract Plant cell, tissue, and organ culture have become a powerful technology for the production of biomass, and for the research and biosynthesis of a variety of secondary metabolites. In this work, we report an efficient method for friable callus induction applied to the medicinal cactus Coryphantha macromeris, its kinetic behavior, and its phytochemical profile, assessed at the maximum biomass production phase. Callus cultures were obtained from stem discs inoculated on Murashige and Skoog medium (MS) supplemented with 6-benzylaminopurine (BAP; 2.2 µM) and picloram (4.14 µM). The highest biomass production (20.65 g DW L−1) was achieved at nine weeks of culture. Then, the phytochemical profile was analyzed using Ultra-High-Performance Liquid Chromatography-tandem Mass Spectrometry (UHPLC-PDA-HESI-Orbitrap-MS/MS). Chromatographic and mass spectrometric analyses indicated the presence of 61 metabolites, with 52 being identified. Among these compounds, 11 organic acids, 16 phenolic acids, 8 flavonoids, and 17 metabolites of different classes were identified. Our results could significantly contribute to the current knowledge of tissue culture of cacti species, as well as the potential applications of the in vitro callus culture of C. macromeris. Furthermore, we report the presence of some metabolites in cell culture of cacti species and their fragmentation pattern for the first time.

中文翻译：

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传统药用植物 Coryphantha macromeris (Engelm.) Britton & Rose (Cactaceae) 愈伤组织培养物中的生物质生产和次生代谢物鉴定

摘要 植物细胞、组织和器官培养已成为生物质生产、多种次生代谢产物研究和生物合成的有力技术。在这项工作中，我们报告了一种适用于药用仙人掌 Coryphantha macromeris 的易碎愈伤组织诱导的有效方法，其动力学行为和植物化学特征，在最大生物质生产阶段进行评估。愈伤组织培养物是从接种在 Murashige 和 Skoog 培养基 (MS) 上的茎盘中获得的，该培养基补充有 6-苄氨基嘌呤 (BAP；2.2 µM) 和 picloram (4.14 µM)。在培养 9 周时达到最高的生物量产量（20.65 g DW L-1）。然后，使用超高效液相色谱-串联质谱 (UHPLC-PDA-HESI-Orbitrap-MS/MS) 分析植物化学特征。色谱和质谱分析表明存在 61 种代谢物，其中 52 种被鉴定。在这些化合物中，鉴定出 11 种有机酸、16 种酚酸、8 种黄酮和 17 种不同类别的代谢物。我们的结果可能对仙人掌物种组织培养的当前知识以及 C. macromeris 体外愈伤组织培养的潜在应用有重大贡献。此外，我们首次报告了仙人掌物种细胞培养物中某些代谢物的存在及其破碎模式。我们的结果可能对仙人掌物种组织培养的当前知识以及 C. macromeris 体外愈伤组织培养的潜在应用有重大贡献。此外，我们首次报告了仙人掌物种细胞培养物中某些代谢物的存在及其破碎模式。我们的结果可能对仙人掌物种组织培养的当前知识以及 C. macromeris 体外愈伤组织培养的潜在应用有重大贡献。此外，我们首次报告了仙人掌物种细胞培养物中某些代谢物的存在及其破碎模式。