Phagocytosis is a basic immune response to the pathogens invading. Immunosuppression may occur in diseases like sepsis and cancer, and cause a low phagocytic ability of phagocytes. High mobility group protein B1 (HMGB1) is a DNA chaperone which is closely related to the phagocytosis. Nonetheless, its influence on phagocytosis is still controversial. We found that paeonol could inhibit the translocation of HMGB1 from the nucleus to the cytoplasm, it may have an impact on phagocytosis. In the present study, we performed in vivo and in vitro experiments to investigate the influence of paeonol on phagocytosis. Zymosan was used to test the phagocytic function of macrophages. Our results showed that paeonol promotes the phagocytosis of macrophages through confining HMGB1 to the nucleus. Through interacting with P53, the nuclear HMGB1 keep it in the nucleus and decrease the negative influence of P53 on the phosphorylation of Focal Adhesion Kinase (FAK). The increasing of phosphorylated FAK promotes the formation of pseudopod and enhances the phagocytic ability of macrophages.

吞噬作用是对病原体入侵的基本免疫反应。免疫抑制可能发生在败血症和癌症等疾病中，并导致吞噬细胞的吞噬能力低下。高迁移率族蛋白B1（HMGB1）是一种与吞噬作用密切相关的DNA分子伴侣。尽管如此，其对吞噬作用的影响仍存在争议。我们发现丹皮酚可以抑制HMGB1从细胞核到细胞质的转运，可能对吞噬作用有影响。在本研究中，我们在体内和体外进行了实验研究丹皮酚对吞噬作用的影响。酵母聚糖用于测试巨噬细胞的吞噬功能。我们的结果表明，丹皮酚通过将HMGB1限制在细胞核中来促进巨噬细胞的吞噬作用。通过与P53相互作用，核HMGB1使其保持在细胞核中，并减少了P53对局灶性粘附激酶（FAK）磷酸化的负面影响。磷酸化FAK的增加促进假足的形成并增强巨噬细胞的吞噬能力。