Host response to an implanted biomaterial is a complex process involving microscopic changes in extracellular matrix (ECM) composition. Reliable pathology analysis is imperative for accurate assessment of the tissue response to an implanted device. Plastic histology is commonly used for histology evaluation of medical devices to assess the device-tissue interface; however, this technique is prone to variable staining that can confound histology interpretation. Appropriately, we propose using transmission electron microscopy (TEM) to confirm histologic ECM findings in order to provide sufficient host-response data.

Tissue response to an absorbable shape memory polymer intravascular occlusion device with a nitinol wire backbone was evaluated. Representative plastic-embedded, micro-ground sections from 30-day, 60-day, and 90-day timepoints were analyzed. ECM regions were selected, and ultrathin sections were created for TEM evaluation. Histological changes in ECM composition were compared for light microscopy (LM) and TEM findings; specifically, TEM fibrillary patterns for collagen and fibrin were used to confirm LM results.

Throughout this study, LM reveals inconsistent staining in plastic-embedded sections. TEM, on the other hand, provides clear insight into the tissue response by morphologically discerning distinct fibrillary patterns within ECM structures; loose to dense collagen surrounds the implant as fibrin degrades, demonstrating progression of postimplant ECM maturation. Moreover, TEM serves as a definitive method for confirming tissue substrate morphology when LM findings prove ambiguous.

宿主对植入的生物材料的反应是一个复杂的过程，涉及细胞外基质（ECM）组成的微观变化。为了准确评估组织对植入设备的反应，必须进行可靠的病理分析。塑性组织学通常用于医疗设备的组织学评估，以评估设备与组织的界面。但是，这种技术易于染色，会混淆组织学解释。适当地，我们建议使用透射电子显微镜（TEM）确认组织学ECM发现，以便提供足够的宿主反应数据。

评价了对具有镍钛诺金属丝骨架的可吸收形状记忆聚合物血管内闭塞装置的组织反应。分析了来自30天，60天和90天时间点的代表性塑料包埋的微型地面部分。选择ECM区域，并创建超薄切片用于TEM评估。比较ECM组成的组织学变化，以进行光学显微镜（LM）和TEM观察；具体而言，使用胶原蛋白和纤维蛋白的TEM原纤维模式来确认LM结果。

在整个研究过程中，LM揭示了塑料嵌入切片中的染色不一致。另一方面，透射电镜通过在形态上辨别ECM结构内不同的原纤维形态，从而提供了对组织反应的清晰见解；当纤维蛋白降解时，松散至致密的胶原蛋白会围绕着植入物，表明植入后ECM成熟的进程。此外，当LM发现不明确时，TEM是确定组织基质形态的确定方法。