An integrated treatment coupling ultrasonic and hydrothermal pretreatments with sequential alkali post-extractions was performed to isolate and characterize hemicelluloses from perennial ryegrass and improve the enzymatic hydrolysis efficiency of cellulose. The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra. Results showed that more than 90% of the original hemicelluloses in ryegrass were released during the integrated treatment and all hemicellulosic fractions obtained were mainly composed of ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans, galactoanrabinoxylans and β-glucans. In addition, the effective removal of amorphous hemicelluloses and lignin significantly increased the cellulose enzymatic hydrolysis rate of ryegrass from 43.8 to 91.1%. These results provided new insights into the collaborative utilization of hemicelluloses and cellulose in ryegrass.

进行了综合处理，将超声波和水热预处理与连续的碱后萃取相结合，以从多年生黑麦草中分离和表征半纤维素并提高纤维素的酶促水解效率。通过凝胶渗透色谱，高效阴离子交换色谱，水热上清液和水热预处理黑麦草制得的水溶性和碱溶性半纤维素的得率，化学组成和结构以及纤维素的酶水解效率，进行了全面研究。傅立叶变换红外光谱和核磁共振光谱。O-甲基-β-葡糖醛酸）-β-木聚糖，半乳阿拉伯糖基木聚糖和β-葡聚糖。此外，有效去除非晶态半纤维素和木质素显着提高了黑麦草的纤维素酶水解率，从43.8％增至91.1％。这些结果为黑麦草中半纤维素和纤维素的协同利用提供了新的见解。