In this work, we present a novel ex situ modification of bacterial cellulose (BC) polymer, that significantly improves its ability to absorb water after drying. The method involves a single inexpensive and easy-to-perform process of BC crosslinking, using citric acid along with catalysts, such as disodium phosphate, sodium bicarbonate, ammonium bicarbonate or their mixtures. In particular, the mixture of disodium phosphate and sodium bicarbonate was the most promising, yielding significantly greater water capacity (over 5 times higher as compared to the unmodified BC) and slower water release (over 6 times as compared to the unmodified BC). Further, our optimized crosslinked BC had over 1.5x higher water capacity than modern commercial dressings dedicated to highly exuding wounds, while exhibiting no cytotoxic effects against fibroblast cell line L929 in vitro. Therefore, our novel BC biomaterial may find application in super-absorbent dressings, designed for chronic wounds with imbalanced moisture level.

在这项工作中，我们提出了一种非原著小说细菌纤维素（BC）聚合物的改性，可显着提高干燥后的吸水能力。该方法涉及使用柠檬酸和催化剂如磷酸氢二钠，碳酸氢钠，碳酸氢铵或其混合物的单一廉价且易于执行的BC交联方法。特别地，磷酸二氢钠和碳酸氢钠的混合物是最有前途的，产生显着更大的水容量（与未改性的BC相比高5倍）和较慢的水释放（与未改性的BC相比超过6倍）。此外，与专门用于高度渗出伤口的现代商业敷料相比，我们优化的交联BC的水容量高出1.5倍以上，同时在体外对成纤维细胞L929没有表现出细胞毒性作用。因此，我们的新型BC生物材料可能会在超吸收性敷料中找到应用，该敷料专为水分含量不均衡的慢性伤口而设计。