Studies on the effects of an electromagnetic field on the activity of recombinant luciferase in the luciferase–luciferin–ATP-Mg²⁺ system were conducted. The enzymatic activity was evaluated by the measurements of biochemiluminescence intensity with a standard chemiluminometer. Frequencies that effectively caused irreversible changes in the enzyme activity were identified. The intensity of chemiluminescence (I_cl), after it reached the stationary level, and the time constant of the intensity decay, after cessation of the stationary luminescence due to ATP depletion, were the criteria of the luciferase activity. I₀ sharply increased from (250 ± 47) imp/s to (1250 ± 75) imp/s compared to the control under exposure to an electromagnetic field of 6 Hz. At the same time, the duration of the stationary luminescence decreased by approximately five times (from 30 to 5.5 min). The decay of luminescence, τ, accelerated significantly (by nine times relative to the control). An opposite effect of other experimentally chosen frequencies of the electromagnetic field was obtained. As an example, upon exposure to the electromagnetic field with frequencies of 12, 48, and 96 Hz, the luciferase activity decreased by 2 times (at 48 and 96 Hz) and by 4 times (at 12 Hz) compared to the control. Luciferase activity did not differ from the control after exposure to a field of 24 Hz.

研究了电磁场对萤光素酶-萤光素-ATP-Mg ²⁺系统中重组萤光素酶活性的影响。通过用标准化学发光计测量生物化学发光强度来评估酶活性。确定了有效引起酶活性不可逆变化的频率。萤光素酶活性的标准是化学发光强度（I _cl）达到稳定水平后，强度衰减的时间常数（由于ATP耗尽而停止稳定发光后）。我₀与暴露在6 Hz电磁场下的对照相比，从（250±47）imp / s急剧增加到（1250±75）imp / s。同时，固定发光的持续时间减少了大约五倍（从30分钟到5.5分钟）。发光的衰减τ显着加速（相对于对照而言是9倍）。获得了其他实验选择的电磁场频率的相反效果。例如，与对照相比，暴露于频率为12、48和96 Hz的电磁场时，荧光素酶活性降低了2倍（在48和96 Hz下）和4倍（在12 Hz下）。暴露于24 Hz电场后，荧光素酶活性与对照无差异。