Abstract

Composition of phospholipids was examined in embryogenic and non-embryogenic cell lines of Siberian larch (Larix sibirica Ledeb.). Significant differences in the content and composition of phospholipids were revealed between the cell lines. Phosphatidylcholines (PC) and phosphatidylethanolamines (PE) were predominant phospholipids in embryogenic cell lines, whereas non-embryogenic cell lines featured a high content of phosphatidic acid (>64% of total phospholipids). The PC/PE ratio known to characterize the membrane structure and function was 1.4–3.8 times higher for embryogenic cell lines than for non-embryogenic lines; this was probably caused by the involvement of these phospholipids in tissue differentiation. The content of phosphatidylinositols differed significantly for embryogenic and non-embryogenic cell lines, where it equaled to 0.50–1.06 and 0.17–0.34 mg/g dry wt, respectively. The observed variations in the composition of phospholipids between the cell lines with different embryogenic potential provide evidence for the important role of these lipids in embryogenesis at the stage of embryo formation.

中文翻译：

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落叶松（Larix sibirica Ledeb）胚胎和非胚胎细胞系的磷脂。

摘要

在西伯利亚落叶松（Larix sibirica）的胚发生和非胚发生细胞系中检查了磷脂的组成Ledeb。）。在细胞系之间揭示了磷脂含量和组成上的显着差异。磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE）是胚发生细胞系中的主要磷脂，而非胚发生细胞系具有高含量的磷脂酸（> 64％的总磷脂）。已知可表征膜结构和功能的PC / PE比是非胚性细胞系的1.4-3.8倍；这可能是由于这些磷脂参与组织分化所致。磷脂酰肌醇的含量对于胚胎发生和非胚胎发生细胞系均存在显着差异，分别等于0.50–1.06和0.17–0.34 mg / g干重。