Although classical metabolic engineering strategies have succeeded in developing microbial strains capable of producing desired bioproducts, metabolic imbalance resulting from extensive genetic manipulation often leads to decreased productivity. Thus, abiotic strategies for improving microbial production performance can be an alternative to overcome drawbacks arising from intensive metabolic engineering. Herein, we report a promising abiotic method for enhancing lycopene production by UV-C irradiation using a radiation-resistant ΔcrtLm/crtB⁺dxs⁺Deinococcus radiodurans R1 strain. First, the onset of UV irradiation was determined through analysis of the expression of 11 genes mainly involved in the carotenoid biosynthetic pathway in the ΔcrtLm/crtB⁺dxs⁺D. radiodurans R1 strain. Second, the effects of different UV wavelengths (UV-A, UV-B, and UV-C) on lycopene production were investigated. UV-C irradiation induced the highest production, resulting in a 69.9% increase in lycopene content [64.2 ± 3.2 mg/g dry cell weight (DCW)]. Extended UV-C irradiation further enhanced lycopene content up to 73.9 ± 2.3 mg/g DCW, a 95.5% increase compared to production without UV-C irradiation (37.8 ± 0.7 mg/g DCW).

中文翻译：

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通过 UV-C 照射增强耐辐射奇异球菌 R1 中的番茄红素产量。

尽管经典的代谢工程策略已成功开发出能够生产所需生物产品的微生物菌株，但广泛的基因操作导致的代谢失衡通常会导致生产力下降。因此，提高微生物生产性能的非生物策略可以作为克服密集代谢工程带来的缺点的替代方法。在此，我们报告了一种有前途的非生物方法，使用耐辐射的 Δ crtLm/crtB ⁺ dxs ⁺ Deinococcus radiodurans R1 菌株通过 UV-C 照射提高番茄红素的产量。首先，通过分析 Δ crtLm/crtB中主要参与类胡萝卜素生物合成途径的 11 个基因的表达来确定紫外线照射的开始⁺ dxs ⁺ D. radiodurans R1 菌株。其次，研究了不同紫外线波长（UV-A、UV-B 和 UV-C）对番茄红素生产的影响。UV-C 照射诱导的产量最高，导致番茄红素含量增加 69.9% [64.2 ± 3.2 mg/g 细胞干重 (DCW)]。延长的 UV-C 照射进一步提高了番茄红素含量，高达 73.9 ± 2.3 mg/g DCW，与没有 UV-C 照射的生产（37.8 ± 0.7 mg/g DCW）相比增加了 95.5%。