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Alteration of lung and gut microbiota in IL-13-transgenic mice simulating chronic asthma.
Journal of Microbiology and Biotechnology ( IF 2.8 ) Pub Date : 2020-10-08 , DOI: 10.4014/jmb.2009.09019
Kyoung-Hee Sohn 1, 2 , Min-Gyung Baek 3 , Sung-Mi Choi 3 , Boram Bae 1 , Ruth Yuldam Kim 1 , Young-Chan Kim 1 , Hye-Young Kim 1, 4 , Hana Yi 3, 5 , Hye-Ryun Kang 1, 6
Affiliation  

Increasing evidence suggests a potential role of microbial colonization in the inception of chronic airway diseases. However, it is not clear whether the lung and gut microbiome dysbiosis is coincidental or a result of mutual interaction. In this study, we investigated the airway microbiome in interleukin 13 (IL-13)-rich lung environment and related alterations of the gut microbiome. IL-13-overexpressing transgenic (TG) mice presented enhanced eosinophilic inflammatory responses and mucus production, together with airway hyperresponsiveness and subepithelial fibrosis. While bronchoalveolar lavage fluid and cecum samples obtained from 10-week-old IL-13 TG mice and their C57BL/6 wild type (WT) littermates showed no significant differences in alpha diversity of lung and gut microbiome, they presented altered beta diversity in both lung and gut microbiota in the IL-13 TG mice compared to the WT mice. Lung-specific IL-13 overexpression also altered the composition of the gut as well as the lung microbiome. In particular, IL-13 TG mice showed an increased proportion of Proteobacteria and Cyanobacteria and a decreased amount of Bacteroidetes in the lungs, and depletion of Firmicutes and Proteobacteria in the gut. The patterns of polymicrobial interaction within the lung microbiota were different between WT and IL-13 TG mice. For instance, in IL-13 TG mice lung Mesorhyzobium significantly affected the alpha diversity of both lung and gut microbiomes. In summary, chronic asthma-like pathologic changes can alter the lung microbiota and affect the gut microbiome. These finding suggest that the lung-gut microbial axis might work actually in asthma.

中文翻译:

模拟慢性哮喘的 IL-13 转基因小鼠肺和肠道微生物群的改变。

越来越多的证据表明微生物定植在慢性气道疾病的发生中具有潜在作用。然而,尚不清楚肺和肠道微生物群失调是巧合还是相互作用的结果。在这项研究中,我们研究了富含白细胞介素 13 (IL-13) 的肺部环境中的气道微生物组和肠道微生物组的相关变化。IL-13 过表达转基因 (TG) 小鼠表现出增强的嗜酸性粒细胞炎症反应和粘液产生,以及气道高反应性和上皮下纤维化。虽然从 10 周大的 IL-13 TG 小鼠及其 C57BL/6 野生型 (WT) 同窝小鼠获得的支气管肺泡灌洗液和盲肠样本显示肺和肠道微生物组的α多样性没有显着差异,与 WT 小鼠相比,他们在 IL-13 TG 小鼠的肺和肠道微生物群中呈现出改变的 β 多样性。肺特异性 IL-13 过表达也改变了肠道和肺微生物组的组成。特别是,IL-13 TG 小鼠表现出增加的比例变形菌蓝藻以及肺中拟杆菌的数量减少,以及肠道中厚壁菌变形菌的消耗。WT 和 IL-13 TG 小鼠肺微生物群内多种微生物相互作用的模式不同。例如,在 IL-13 TG 小鼠中,肺中生菌显着影响肺和肠道微生物组的 alpha 多样性。总之,慢性哮喘样病理变化可以改变肺部微生物群并影响肠道微生物群。这些发现表明,肺-肠微生物轴实际上可能在哮喘中发挥作用。
更新日期:2020-10-20
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