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Acroeimeria lineri (McAllister, Upton, Freed, 1988) Paperna and Landsberg, 1989 in Mediterranean Geckos (Hemidactylus turcicus): Oocyst Morphometrics, Endogenous Developmental Stages, and Molecular Sequencing Support its Placement into Acroeimeria
Journal of Parasitology ( IF 1.3 ) Pub Date : 2020-10-16 , DOI: 10.1645/18-120
A. S. Abdel-Baki 1 , H. M. Abdel-Haleem 1 , D. W. Duszynski 2 , S. Al-Quraishy 3 , L. Mansour 3
Affiliation  

Between June 2016 and June 2019, we surveyed 62 Mediterranean geckos, Hemidactylus turcicus, from Abu Rawash, Giza, Egypt, for the presence of endoparasites. In June 2016, we found 3 individuals to be infected with Eimeria lineri. We studied the morphology and inner structures of its sporulated oocysts, and the locations of its intestinal endogenous stages. We also extracted genomic DNA from these sporulated oocysts and successfully sequenced a 632-bp fragment of the 18S rRNA gene. Phylogenetic analyses using this partial sequence allowed us to support previous studies that assigned E. lineri to the genus Acroeimeria. Our consensus sequence was used to query similar 18S rDNA sequences from GenBank, and 14 sequences were selected. The phylogenetic analysis inferred by maximum likelihood and Bayesian inference methods gave similar results, as both separated the sequences into 2 clades: (1) a monophyletic group of Goussia species (from fish); and (2) a strongly supported clade that separated 4 Choleoeimeria species from a polyphyletic group of species that clustered A. lineri with 3 other Acroeimeria species and 3 Eimeria species from lizards, including Eimeria tiliquae from Tiliqua rugosa (Gray, 1825), Eimeria tokayae from Gecko gecko (L., 1758), and Eimeria eutropidis from Eutropis macularia (Blyth, 1853). Our study supports the placement of E. lineri into the Acroeimeria and contributes additional life history information toward understanding the evolutionary origin of the Eimeria-like species that have sporocysts without Stieda bodies in their oocysts and that infect saurian reptiles. We also support the concept that several traits (morphological, endogenous, and gene sequences) are both necessary and important for authors to include when making generic reassignments within the eimeriid coccidia.



中文翻译:

Acroeimeria lineri(McAllister,Upton,Freed,1988)Paperna and Landsberg,1989在地中海壁虎(Hemidactylus turcicus):卵囊形态计量学,内源性发育阶段和分子测序支持将其放入Acroeimeria

在2016年6月至2019年6月之间,我们对来自埃及吉萨的Abu Rawash的62只地中海壁虎Hemidactylus turcicus进行了调查,以调查是否存在内寄生虫。2016年6月,我们发现3个人感染了艾美叶虫。我们研究了孢子形成的卵囊的形态和内部结构,以及肠道内生阶段的位置。我们还从这些孢子形成的卵囊中提取了基因组DNA,并成功地对18S rRNA基因的632-bp片段进行了测序。系统发育分析使用这部分序列使我们支持先前的研究,将大肠杆菌(E. lineri)分配到属属。我们的共有序列用于查询相似的18S rDNA来自GenBank的序列,并选择了14个序列。通过最大似然法和贝叶斯推断法进行的系统发育分析得出了相似的结果,因为它们都将序列分为两个进化枝:(1)一类古斯种(来自鱼类);和(2)一个强烈支持进化枝该分离4个Choleoeimeria物种从物种的多元组群集A. lineri用3种其他Acroeimeria物质和3-艾美球虫从蜥蜴物种,包括艾美球虫tiliquaeTiliqua藿香(灰色,1825),艾美耳球虫tokayae来自壁虎壁虎(L.,1758),和艾美耳球虫eutropidisEutropis macularia(布莱思,1853年)。我们的研究支持将线虫放入鹰cro中,并为了解像蠕虫一样在孢囊中没有孢子体的孢子囊的艾美球虫样物种的进化起源提供更多的生活史信息,并感染蜥蜴爬行动物。我们还支持这样的概念,即当在球虫科球菌内进行一般性重新分配时,包括作者在内的几个特征(形态,内源和基因序列)既必要又重要。

更新日期:2020-10-17
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