This study evaluates the hemostatic properties of tantalum-containing mesoporous bioactive glasses (Ta-MBGs) through a suite of in-vitro methods: hemolysis percentage, zeta potential, blood coagulation assays (Activated Partial Thromboplastin Time – APTT and Prothrombin Time - PT) and cytotoxicity tests. Five compositions of Ta-MBG, with x mol% Ta₂O₅ added to the glass series (80-x)SiO₂-15CaO-5P₂O₅-xTa₂O₅ where x=0 (0Ta), x=0.5 (0.5Ta), x=1 (1Ta), x=5 (5Ta), and x=10 (10Ta) mol%, were synthesised. The hemostatic potential of all the Ta-MBGs was confirmed by their negative zeta potential (–23 to –31 mV), which enhances the intrinsic pathway of blood coagulation. The hemolysis percentages of all Ta-MBGs except 10Ta showed statistically significant reductions compared to the same experiments carried out both in the absence of a sample (‘no treatment’ group) and in the presence of 10Ta. These observations validate the consideration of Ta-MBGs as hemostatic agents as they do not cause significant lysis of red blood cells. Cytotoxicity analysis revealed that Ta-MBGs had no effect on bovine fibroblast viability. Furthermore, a reduction in both APTT (a test to evaluate the intrinsic pathway of coagulation) and PT (a test to evaluate the extrinsic pathway) signified enhancement of hemostasis: 5Ta caused a significant reduction in APTT compared to ‘no treatment’, 1Ta and 10Ta and a significant reduction in PT compared to 0Ta. Therefore, we conclude that 5mol% of Ta optimised the hemostatic properties of these mesoporous bioactive glasses.

本研究通过一系列体外方法评估含钽介孔生物活性玻璃 (Ta-MBG) 的止血特性：溶血百分比、zeta 电位、凝血测定（活化部分凝血活酶时间 - APTT 和凝血酶原时间 - PT）和细胞毒性试验。Ta-MBG 的五种成分，其中x mol% Ta ₂ O ₅添加到玻璃系列 (80- x )SiO ₂ -15CaO-5P ₂ O ₅ - x Ta ₂ O ₅其中x =0 (0Ta), x = 0.5 (0.5Ta), x =1 (1Ta), x=5 (5Ta)，且x=10 (10Ta) mol%，合成。所有 Ta-MBG 的止血电位均由其负 zeta 电位（–23 至–31 mV）证实，这增强了血液凝固的内在途径。与在没有样品（“未处理”组）和存在 10Ta 的情况下进行的相同实验相比，除 10Ta 之外的所有 Ta-MBG 的溶血百分比显示出统计学上显着的降低。这些观察结果验证了将 Ta-MBG 作为止血剂的考虑，因为它们不会导致红细胞显着溶解。细胞毒性分析表明，Ta-MBGs 对牛成纤维细胞的活力没有影响。此外，APTT（评估凝血内在途径的测试）和 PT（评估外在途径的测试）的减少表明止血的增强：与“未处理”、1Ta 和 10Ta 相比，5Ta 导致 APTT 显着降低，与 0Ta 相比，PT 显着降低。因此，我们得出结论，5mol% 的 Ta 优化了这些介孔生物活性玻璃的止血性能。