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An improved comprehensive strategy for deep and quantitative N-glycomics based on optimization of sample preparation, isotope-based data quality control and quantification, new N-glycan libraries and new algorithms
bioRxiv - Biochemistry Pub Date : 2022-10-13 , DOI: 10.1101/2020.10.15.340638
Yudong Guan , Jiaxiang Hu , Weiqian Cao , Wencong Cui , Fan Yang , Christoph Krisp , Ling Lin , Min Zhang , Hannah Voss , Raphael Schuster , Guoquan Yan , Marceline Manka Fuh , Morten Thaysen-Andersen , Nicolle H. Packer , Huali Shen , Pengyuan Yang , Hartmut Schlüter

The authors have withdrawn their manuscript because the authors need to re-organize the data and writing, meanwhile more experimental evidence from human cellular N-glycome will be added to support their viewpoints. In this preprint, only one human cell line, Acute Promyelocytic Leukemia (APL) cells, was deeply investigated to show the N-glycome landscape. To provide the shared features of human N-glycome, more human cells should be investigated. Therefore, the authors do not wish this work to be cited as reference for the project. If you have any questions, please contact the corresponding author.

中文翻译:

基于样品制备优化、基于同位素的数据质量控制和定量、新的 N-聚糖库和新算法的改进的深度和定量 N-糖组学综合策略

作者撤回了他们的手稿,因为作者需要重新组织数据和写作,同时将添加更多来自人类细胞 N-糖组的实验证据来支持他们的观点。在本预印本中,仅对一种人类细胞系——急性早幼粒细胞白血病 (APL) 细胞进行了深入研究,以展示 N-糖组景观。为了提供人类 N-糖组的共同特征,应该研究更多的人类细胞。因此,作者不希望这项工作被引用作为该项目的参考。如有任何问题,请联系通讯作者。
更新日期:2022-10-13
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