Fusarium head blight (FHB), a devastating wheat disease, results in loss of yield and production of mycotoxins including deoxynivalenol (DON) in infected grains. DON is harmful to human and animal health and facilitates the spread of FHB symptoms. Its conversion into DON-3-glucoside (D3G) by UDP-glycosyltransferases (UGTs) is correlated with FHB resistance, and only few gene members in wheat have been investigated. Here, Fusarium graminearum and DON-induced TaUGT6 expression in the resistant cultivar Sumai 3 was cloned and characterized. TaUGT6::GFP was subcellularly located throughout cells. Purified TaUGT6 protein could convert DON into D3G to some extent in vitro. Transformation of TaUGT6 into Arabidopsis increased root tolerance when grown on agar plates containing DON. Furthermore, TaUGT6 overexpression in wheat showed improved resistance to Fusarium spread after F. graminearum inoculation. Overall, this study provides useful insight into a novel UGT gene for FHB resistance in wheat.

镰刀菌枯萎病（FHB）是一种毁灭性的小麦病，导致受感染谷物中霉菌毒素（包括脱氧雪腐酚（DON））的产量和产量损失。DON对人类和动物健康有害，并促进FHB症状的传播。它通过UDP-糖基转移酶（UGT）转化为DON-3-葡萄糖苷（D3G）与FHB抗性相关，并且仅研究了小麦中的少数基因成员。这里，禾谷镰刀菌 和DON引起的 TaUGT6克隆并鉴定了抗性品种Sumai 3中的表达。TaUGT6 :: GFP位于整个细胞的亚细胞位置。纯化的TaUGT6蛋白可以在一定程度上将DON转化为D3G体外。转型TaUGT6 进入 拟南芥在含有DON的琼脂平板上生长时，增加了根的耐受性。此外，TaUGT6 小麦中的过表达显示出增强的抗病性 镰刀菌 之后传播 F。 禾本科接种。总体而言，这项研究为小说提供了有益的见解UGT 小麦FHB抗性基因。