In this paper, an approach for the monitoring of biotechnological process kinetics is proposed. The kinetics of each process state variable is presented as a function of two time-varying unknown parameters. For their estimation, a general software sensor is derived with on-line measurements as inputs that are accessible in practice. The stability analysis with a different number of inputs shows that stability can be guaranteed for fourth- and fifth-order software sensors only. As a case study, the monitoring of the kinetics of processes carried out in stirred tank reactors is investigated. A new tuning procedure is derived that results in a choice of only one design parameter. The effectiveness of the proposed procedure is demonstrated with experimental data from Bacillus subtilis fed-batch cultivations.

中文翻译：

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生物工艺过程动力学的自适应监测

本文提出了一种监测生物技术过程动力学的方法。每个过程状态变量的动力学表示为两个随时间变化的未知参数的函数。对于它们的估计，使用在线测量得出的通用软件传感器作为输入，可以在实践中访问。输入数量不同时的稳定性分析表明，只能保证四阶和五阶软件传感器的稳定性。作为案例研究，研究了搅拌釜反应器中过程动力学的监测。推导了一种新的调整过程，该过程导致只能选择一个设计参数。枯草芽孢杆菌补料分批培养的实验数据证明了该方法的有效性。