One of the most common evolutionary transitions in angiosperms is the reproductive change from outcrossing to selfing, commonly associated with changes in floral biology and genetic diversity. Here, we aim to test whether self-compatibility leads to a reduction of floral traits and genetic diversity. For this, we experimentally estimate levels of self-compatibility, measure three floral traits and estimate four genetic diversity parameters using nine microsatellites in nine Calceolaria species. Our analysis indicated that four of the study species were self-incompatible. In addition, we found that self-compatible species did not show a reduction in floral traits size, but rather displayed larger corolla and elaiophore areas. Our analyses of genetic diversity identified larger allele number and observed heterozygosity in selfers than in outcrossers, but did not find larger inbreeding in the self-compatible species. Even though our results contradict our expectations, in the case of Calceolaria, their high dependence on only two genera of oil-bees puts the genus in a vulnerable reproductive position, probably facilitating the evolution of reproductive assurance mechanisms in the absence of pollinators. As a result, plants maintain their pollinator attraction traits while evolving the ability to self, possibly in a delayed way.

中文翻译：

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奖励性油菜种类与形态或遗传多样性减少无关的自相容性

被子植物最常见的进化转变之一是从异交到自交的生殖变化，通常与花生物学和遗传多样性的变化有关。在这里，我们旨在测试自我兼容性是否会导致花卉性状和遗传多样性的降低。为此，我们实验性地估计了自我相容性的水平，测量了三种花卉性状，并使用了九个Calceolaria中的九个微卫星来估计了四个遗传多样性参数。种类。我们的分析表明，有四个研究物种是自我不相容的。此外，我们发现自相容物种没有显示出花性状大小的减少，而是显示出较大的花冠和e部区域。我们对遗传多样性的分析确定了比异交者更大的等位基因数量，并且在自交者中观察到了杂合性，但是在自相容物种中没有发现更大的近交。即使我们的结果与我们的预期相抵触，但对于Calceolaria，由于它们仅依赖两个油蜂属，因此该属处于脆弱的生殖位置，可能在没有授粉媒介的情况下促进了生殖保证机制的进化。结果，植物在保持自身授粉能力的同时，还可能以延迟的方式进化了自身的能力。