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Obtaining, Evaluation, and Optimization of Doxycycline-Loaded Microparticles Intended for the Local Treatment of Infectious Arthritis
Coatings ( IF 3.4 ) Pub Date : 2020-10-17 , DOI: 10.3390/coatings10100990
Maria Minodora Marin , Mădălina Georgiana Albu Kaya , Horia Iovu , Cristina Elena Stavarache , Ciprian Chelaru , Rodica Roxana Constantinescu , Cristina-Elena Dinu-Pîrvu , Mihaela Violeta Ghica

Compared to the classical systemic administration, the local drug release has some advantages, such as lack of systemic toxicity and associated side effects, increased patient compliance, and a low rate of bacterial resistance. Biopolymers are widely used to design sustained drug delivery systems and biomaterials for tissue engineering. Type II collagen is the indispensable component in articular cartilage and plays a critical role in the growth and proliferation process of chondrocytes. Thus, type II collagen has drawn more attention and interest in the treatment and research of the cartilage regeneration. The aim of this study was to obtain, characterize, and optimize the microcapsules formulation based on type II collagen, sodium alginate, and sodium carboxymethyl cellulose loaded with doxycycline as an antibiotic model drug that could be incorporated further in hydrogels to improve the localized therapy of septic arthritis. The new synthesized microcapsules were assessed by spectral (FT-IR), morphological (optical microscopy), and biological analysis (enzymatic biodegradation, antimicrobial activity). The size distribution of the obtained microcapsules was determined using optical microscopy. The drug encapsulation efficiency was also determined. To optimize the microcapsules’ composition, some physical-chemical and biological analyses were subjected to an optimization technique based on experimental design, response surface methodology, and the Taguchi technique, and the adequate formulations were selected. The results obtained recommend these new microcapsules as promising drug systems to be further incorporated in type II collagen hydrogels used for septic arthritis.

中文翻译:

用于感染性关节炎局部治疗的多西环素负载微粒的获取,评估和优化

与传统的全身给药相比,局部药物释放具有一些优势,例如缺乏全身毒性和相关的副作用,患者顺应性增加以及细菌耐药率低。生物聚合物被广泛用于设计用于组织工程的持续药物输送系统和生物材料。II型胶原蛋白是关节软骨中必不可少的成分,并且在软骨细胞的生长和增殖过程中起着至关重要的作用。因此,II型胶原在软骨再生的治疗和研究中引起了更多的关注和兴趣。这项研究的目的是获得,表征和优化基于II型胶原蛋白,海藻酸钠,载有强力霉素的羧甲基纤维素钠作为抗生素模型药物,可以进一步掺入水凝胶中以改善化脓性关节炎的局部治疗。通过光谱(FT-IR),形态学(光学显微镜)和生物学分析(酶促生物降解,抗菌活性)评估了新合成的微囊。使用光学显微镜确定获得的微胶囊的尺寸分布。还确定了药物包封效率。为了优化微胶囊的组成,基于实验设计,响应面方法和田口技术,对一些物理化学和生物学分析进行了优化,并选择了合适的制剂。
更新日期:2020-10-17
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