Cochlear hair cells in human beings cannot regenerate after loss; however, those in fish and other lower species can. Recently, the role of inflammation in hair cell regeneration has been attracting the attention of scientists. In the present study, we investigated how suppression of inflammatory factors affects hair cell regeneration and the functional recovery of regenerated hair cells in zebrafish. We killed hair cells in the lateral line of zebrafish larvae with CuSO₄ to induce an inflammatory response and coapplied BRS-28, an anti-inflammatory agent to suppress the inflammation. The recovery of the hair cell number and rheotaxis was slower when CuSO₄ and BRS-28 were coapplied than when CuSO₄ was applied alone. The recovery of hair cell count lagged behind that of the calcium imaging signal during the regeneration. The calcium imaging signal in the neuromasts in the inflammation-inhibited group was weaker than that in the noninflammation-inhibited group at the early stage of regeneration, although it returned to normal at the late stage. Our study demonstrates that suppressing inflammation by BRS-28 delays hair cell regeneration and functional recovery when hair cells are damaged. We suspect that BRS-28 inhibits pro-inflammatory factors and thereby reduces the migration of macrophages to delay the regeneration of hair cells.

中文翻译：

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抑制炎症会延迟斑马鱼幼虫侧线损伤后毛细胞的再生和功能的恢复。

人的耳蜗毛细胞在丢失后无法再生；但是，鱼类和其他低等物种中的那些可以。最近，炎症在毛细胞再生中的作用已引起科学家的注意。在本研究中，我们调查了炎症因子的抑制如何影响斑马鱼的毛细胞再生和再生毛细胞的功能恢复。我们用CuSO ₄杀死了斑马鱼幼虫侧线中的毛细胞，以诱导炎症反应，并共同应用了BRS-28（一种抑制炎症的抗炎剂）。当的CuSO毛细胞数量和rheotaxis的回收率为较慢的₄和BRS-28时相比，硫酸铜被coapplied ₄被单独应用。在再生期间，毛细胞计数的恢复落后于钙成像信号的恢复。在再生的早期，炎症抑制组的神经瘤中的钙显像信号弱于非炎症抑制组，但在后期却恢复正常。我们的研究表明，当毛细胞受损时，通过BRS-28抑制炎症会延迟毛细胞再生和功能恢复。我们怀疑BRS-28会抑制促炎因子，从而减少巨噬细胞的迁移，从而延迟毛细胞的再生。