This study investigated Allium subhirsutum L. (AS) anticancer and antioxidant effects and inhibition of tumor angiogenesis in a murine model of skeletal metastases due to inoculation of Walker 256/B cells. Phytochemical composition of AS extract (ASE) was studied by High Resolution-Liquid Chromatography Mass Spectroscopy (HR-LCMS). Total phenolic and flavonoid contents (TPC and TFC) were determined. In vitro, the antioxidant properties were evaluated by reducing power and antiradical activity against DPPH. Cancer cells’ proliferation, apoptosis, metastatic development and angiogenesis were evaluated using Walker 256/B and MatLyLu cells. The p-coumaric acid was the major phenolic acid (1700 µg/g extract). ASE showed high levels of TPC and TFC and proved potent antioxidant effects. ASE inhibited Walker 256/B and MatLyLu cells’ proliferation (Half-maximal inhibitory concentration: IC₅₀ ≃ 150 µg/mL) and induced apoptosis. In silico and in vivo assays confirmed these findings. ASE effectively acts as a chemo-preventive compound, induces apoptosis and attenuates angiogenesis and osteolytic metastases due to Walker 256/B malignant cells.

中文翻译：

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葱属亚种作为抗氧化和抗癌生物活性分子的潜在来源：HR-LCMS植物化学分析，体外和体内药理研究

这项研究调查了葱属L.（AS）在接种Walker 256 / B细胞引起的骨骼转移小鼠模型中具有抗癌和抗氧化作用，并抑制肿瘤血管生成。通过高分辨率液相色谱质谱法（HR-LCMS）研究了AS提取物（ASE）的植物化学组成。测定了总酚和类黄酮含量（TPC和TFC）。在体外，通过降低对DPPH的功率和抗自由基活性来评估其抗氧化性能。使用Walker 256 / B和MatLyLu细胞评估癌细胞的增殖，凋亡，转移发展和血管生成。对香豆酸是主要的酚酸（1700 µg / g提取物）。ASE显示出高水平的TPC和TFC，并证明了有效的抗氧化作用。ASE抑制Walker 256 / B和MatLyLu细胞的增殖（半最大抑制浓度：IC₅₀ ≃150微克/毫升）和诱导细胞凋亡。在计算机和体内试验中证实了这些发现。ASE有效地充当化学预防化合物，诱导细胞凋亡，并减轻由于Walker 256 / B恶性细胞而引起的血管生成和溶骨性转移。