Abstract

Medicago sativa L. (alfalfa) are studied as potential phytoremediation agents of priority pollutants like polycyclic aromatic hydrocarbons (PAH). However, elucidation of the biochemical mechanisms involved in phytoremediation is a topic to be explored with knowledge gaps. This study aims to identify and classify proteins expressed in the aerial parts of laboratory-cultivated alfalfa in the presence and absence of pyrene, anthracene, and phenanthrene. Soil samples were amended with 100 mg.kg⁻¹ of each PAH (total concentration of 300 ppm) and cultivated with alfalfa plants for 20 days. After this, aerial parts of cultivated plants from each condition were collected for qualitative proteomic analysis (ESI-Q/TOF). The results showed a significant increase (Student’s t-test p < 0.05) of 41.7% in the concentration of proteins from plants grown in PAH-amended substrates, changes in the protein profile, with intense protein bands observed at 40–55, 34, 28, and 15 kDa when compared to the control. A total of 504 proteins were identified and classified into 12 functional categories, highlighting the identification of 11 phytoremediation-related proteins candidates in plants grown in the presence of PAH, with biological functions related to diverse metabolisms involved in the xenobiotics biodegradation (included PAH), glutathione and response to stress.

抽象的

紫花苜蓿（苜蓿）被研究为优先污染物（如多环芳烃）的潜在植物修复剂。然而，阐明与植物修复有关的生化机制是一个存在知识空白的问题。这项研究旨在鉴定和分类在有pyr，蒽和菲存在和不存在的情况下，实验室培养的苜蓿地上部分表达的蛋白质。用100 mg.kg ^-1的每种PAH（总浓度为300 ppm）修改土壤样品，并用苜蓿植物培养20天。此后，收集每种条件下栽培植物的地上部分，进行定性蛋白质组分析（ESI-Q / TOF）。结果显示显着增加（学生t检验p  <0.05），在PAH修饰的底物中生长的植物中的蛋白质浓度为41.7％，蛋白质谱发生变化，与对照相比，在40–55、34、28和15 kDa处观察到强烈的蛋白质带。共鉴定出504种蛋白质并将其分类为12个功能类别，从而突出显示了在PAH存在下生长的植物中11种与植物修复相关的蛋白质的鉴定，其生物学功能与异生物素生物降解（包括PAH）中涉及的多种代谢有关，谷胱甘肽和对压力的反应。