Biogenic metal/metalloid nanoparticles of microbial origin retain a functional biomolecular capping layer that confers structural stability. Little is known about the composition of such capping material. In this study, selenium nanoparticles (SeNPs) synthesized by five different bacterial strains underwent comparative analysis with newly proposed protocols for quantifying the concentration of carbohydrates, proteins and lipids present in capping layers. SeNPs were therefore treated with two different detergents to remove portions of the surrounding caps in order to assess the resulting effects. Capping material quantification was carried out along with the measure of parameters such as hydrodynamic diameter, polydispersity and surface charge. SeNPs from the five strains showed differences in their distinct biomolecule ratios. On the other hand, structural changes in the nanoparticles induced by detergents did not correlate with the amounts of capping matrix removed. Thus, the present investigation suggests a hypothesis to describe capping layer composition of the bacterial SeNPs: some biomolecules are bound more strongly than others to the core metalloid matrix, so that the diverse capping layer components differentially contribute to the overall structural characteristics of the nanoparticles. Furthermore, the application of the approach here in combining quantification of cap‐associated biomolecules with the measurement of structural integrity‐related parameters can give the biogenic nanomaterial field useful information to construct a data bank on biogenically synthesized nanostructures.

中文翻译：

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五种细菌合成的覆盖层的生物分子组成和生物硒纳米颗粒的稳定性

微生物来源的生物金属/准金属纳米颗粒保留了赋予结构稳定性的功能性生物分子覆盖层。对于这种封盖材料的组成知之甚少。在这项研究中，由五种不同细菌菌株合成的硒纳米颗粒（SeNPs）进行了比较分析，并采用了新提出的方案来量化覆盖层中碳水化合物，蛋白质和脂质的浓度。因此，用两种不同的去污剂处理SeNPs，以去除部分周围的盖子，以评估产生的效果。封端材料定量以及参数的测量，例如流体力学直径，多分散性和表面电荷。来自五种菌株的SeNPs在其独特的生物分子比率上显示出差异。另一方面，由去污剂诱导的纳米颗粒的结构变化与去除的封端基质的量不相关。因此，本研究提出了一个假设来描述细菌SeNPs的覆盖层组成：某些生物分子比核心金属更牢固地结合到核心准金属基质上，因此不同的覆盖层组分对纳米颗粒的整体结构特征有不同的贡献。此外，在此方法中将帽相关生物分子的定量与结构完整性相关参数的测量相结合的应用可以为生物纳米材料领域提供有用的信息，以建立有关生物合成纳米结构的数据库。