Lusianthridin, a bioactive component isolated from Dendrobium venustum, has been demonstrated to have many biological properties such as antioxidant and anticancer activities. However, the metabolic profiles remain unknown. This study was carried out to investigate the metabolic profiles of lusianthridin in liver microsomes. Lusianthridin was co‐incubated with liver microsomes in the presence of nicotinamide adenine dinucleotide phosphate and UDP‐glucuronic acid or glutathione at 37°C for 1 h. The incubation samples were analyzed by liquid chromatography combined with electrospray ionization high‐resolution mass spectrometry. The data were acquired and processed. The structures of the metabolites were proposed by comparing their accurate mass and MS² spectra with those of the parent compound. A total of 15 metabolites were detected in vitro, including two phase I and 13 phase II metabolites. The phase I metabolic pathways were oxidation, demethylation and dehydrogenation. The phase II metabolic pathways referred to glucuronidation and glutathione conjugation. The present study provides an overview pertaining to the metabolic profiles of lusianthridin in vitro, which is indispensable for understanding the efficacy and safety of lusianthridin, as well as the herbal medicine D. venustum.

中文翻译：

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液相色谱与电喷雾电离飞行时间质谱联用鉴定大鼠肝微粒体中的菊苣苷代谢物

Lusianthridin，从分离的生物活性组分石斛venustum，已被证明具有许多生物学特性，例如抗氧化剂和抗癌活性。然而，代谢概况仍然未知。进行了这项研究，以研究肝微粒体中香豆精的代谢谱。在存在烟酰胺腺嘌呤二核苷酸磷酸和UDP-葡萄糖醛酸或谷胱甘肽的条件下，将芦硫吡啶与肝微粒体共同孵育1小时。孵育样品通过液相色谱结合电喷雾电离高分辨率质谱分析。数据已获取并处理。通过比较代谢物的准确质量和MS ²提出了代谢物的结构母体化合物的光谱。体外共检测到15种代谢物，包括两种I期和13种II期代谢物。I期代谢途径是氧化，脱甲基和脱氢。II期代谢途径涉及葡萄糖醛酸化和谷胱甘肽结合。本研究提供了有关紫杉醇体外代谢谱的概述，这对于理解紫杉醇以及草药D. venustum的功效和安全性是必不可少的。